Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142290 Pushchino, Moscow Region, Russia.
Biochemistry (Mosc). 2012 Aug;77(8):857-63. doi: 10.1134/S0006297912080068.
In the halophilic aerobic methylotrophic bacterium Methylophaga thalassica, the genes encoding the enzymes for biosynthesis of the osmoprotectant ectoine were shown to be located in operon ectABC-ask. Transcription of the ect-operon was started from the two promoters homologous to the σ(70)-dependent promoter of Escherichia coli and regulated by protein EctR, whose encoding gene, ectR, is transcribed from three promoters. Genes homologous to ectR of methylotrophs were found in clusters of ectoine biosynthesis genes in some non-methylotrophic halophilic bacteria. EctR proteins of methylotrophic and heterotrophic halophiles belong to the MarR-family of transcriptional regulators but form a separate branch on the phylogenetic tree of the MarR proteins.
在嗜盐好氧甲基营养菌 Methylophaga thalassica 中，编码用于合成渗透保护剂海藻糖的酶的基因被证明位于操纵子 ectABC-ask 中。ect 操纵子的转录从与大肠杆菌 σ(70)依赖启动子同源的两个启动子开始，并受蛋白 EctR 的调节，其编码基因 ectR 从三个启动子转录。在一些非甲基营养嗜盐菌的海藻糖生物合成基因簇中发现了与甲基营养菌 ectR 同源的基因。甲基营养型和异养型嗜盐菌的 EctR 蛋白属于 MarR 家族转录调节剂，但在 MarR 蛋白的系统发育树上形成一个单独的分支。
