Mousavi Tahereh, Shahsavar Farhad, Farnia Parisa, Tajik Nader, Soofi Mahbubeh
Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2011 Sep;10(3):189-94.
Analysis of receptor-ligand interactions in the context of diseases necessitates to understand how HLA-KIR genotypes function in diseases. Although CD56+ lymphocytes are derived from multiple lineages, they share a functional association with immunosurviellance and antimicrobial responses. The present study aimed to determine whether KIR phenotype in CD56 lymphocytes and corresponding HLA-class 1 ligands are associated with multidrug resistance tuberculosis (MDR-TB). We compared the frequencies of HLA-C and HLA-BW4 genes, the expression of KIRs 2DL1/2DS1, 2DL2/2DL3, 3DL1, and 2DS4 and the combinations of HLA/KIR in 32 Nifamycin and Isoniazid-resistant TB with those in 68 drug non resistant (NR) sputum smear positive pulmonary TB patients. PCR-SSP and flow cytometry were performed for HLA and KIRs typing, respectively. We showed no significant differences between inhibitory or activating KIRs as well as HLA ligands in MDR TB patients compared with NR-TB . The combinations of inhibitory KIR-HLA ligands in MDR-TB were much more prevalent, but not statistically significant than in NR patients (p=0.07). The frequency of MDR patients with all HLA-C and HLA-BW4 ligands was higher than NR-TB (p<0.009). Conversely, the percentage of MDR patients having only one kind of HLA gene was significantly lower than NR-TB (p<0.01). We conclude that the expression of inhibitory KIRs with corresponding HLA ligands genes, and/or co-existence of three HLA class 1 ligands for inhibitory KIRs may be associated with drug resistance in pulmonary tuberculosis.
在疾病背景下分析受体 - 配体相互作用需要了解HLA - KIR基因型在疾病中的作用机制。虽然CD56 +淋巴细胞来源于多个谱系，但它们在免疫监视和抗菌反应方面具有共同的功能关联。本研究旨在确定CD56淋巴细胞中的KIR表型及相应的HLA - I类配体是否与耐多药结核病（MDR - TB）相关。我们比较了32例对利福霉素和异烟肼耐药的结核病患者与68例药物敏感（NR）痰涂片阳性肺结核患者中HLA - C和HLA - BW4基因的频率、KIRs 2DL1 / 2DS1、2DL2 / 2DL3、3DL1和2DS4的表达以及HLA / KIR的组合情况。分别采用PCR - SSP和流式细胞术进行HLA和KIRs分型。结果显示，与NR - TB患者相比，MDR - TB患者中抑制性或激活性KIRs以及HLA配体之间无显著差异。MDR - TB中抑制性KIR - HLA配体的组合更为普遍，但与NR患者相比无统计学意义（p = 0.07）。具有所有HLA - C和HLA - BW4配体的MDR患者频率高于NR - TB患者（p < 0.009）。相反，仅有一种HLA基因的MDR患者百分比显著低于NR - TB患者（p < 0.01）。我们得出结论，抑制性KIRs与相应HLA配体基因的表达，和/或抑制性KIRs的三种HLA - I类配体共存可能与肺结核的耐药性有关。
