State Key Laboratory of Microbial Metabolism, Marine Biotechnology Laboratory, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
World J Microbiol Biotechnol. 2012 Dec;28(12):3255-60. doi: 10.1007/s11274-012-1136-9. Epub 2012 Aug 4.
The occurrence of harmful algal blooms (HABs) throughout the world has increased and poses a large threat to human health, fishery resources and tourism industries. The genus Alexandrium includes a number of toxic species associated with HABs. Therefore, it is very important to rapidly detect and monitor the harmful algae, such as Alexandrium genus. In this study, a standard curve of plasmid containing 18S rDNA-28S rDNA region from Alexandrium catenella was constructed and 5.8S rDNA sequence served as the primer of the real-time PCR. Cultured A. catenella, Alexandrium affine, Alexandrium lusitanicum and Alexandrium minutum samples were analyzed by real-time PCR using the same set of primers simultaneously. Using microscopy cells counts, 5.8S rDNA copies per cell and total DNA per cell were estimated. This assay method is promising for rapid detection of large number of Alexandrium samples.
有害藻华（HABs）在全球范围内的发生频率不断增加，对人类健康、渔业资源和旅游业构成了巨大威胁。亚历山大藻属包含了一些与 HABs 相关的有毒物种。因此，快速检测和监测有害藻类，如亚历山大藻属，非常重要。在这项研究中，构建了含有亚历山大藻属 18S rDNA-28S rDNA 区的质粒标准曲线，并使用 5.8S rDNA 序列作为实时 PCR 的引物。使用相同的引物，通过实时 PCR 分析培养的亚历山大藻属、亚历山大藻属、亚历山大藻属和亚历山大藻属样本。通过显微镜细胞计数，估计每个细胞的 5.8S rDNA 拷贝数和每个细胞的总 DNA 量。该检测方法有望快速检测大量的亚历山大藻属样本。
