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qRT-PCR: a method and its difficulties.

作者信息

Bollmann Franziska, Casper Ingrid, Henke Jenny, Pautz Andrea

出版信息

Naunyn Schmiedebergs Arch Pharmacol. 2012 Oct;385(10):949-51. doi: 10.1007/s00210-012-0786-3. Epub 2012 Aug 4.

DOI:10.1007/s00210-012-0786-3
PMID:22864909
Abstract
摘要

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BMC Res Notes. 2012 May 7;5:220. doi: 10.1186/1756-0500-5-220.
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Tissue-specific selection of stable reference genes for real-time PCR normalization in an obese rat model.
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Biomed Res Int. 2018 Jul 9;2018:6125706. doi: 10.1155/2018/6125706. eCollection 2018.
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Easy-to-use strategy for reference gene selection in quantitative real-time PCR experiments.定量实时PCR实验中参考基因选择的易用策略。
Naunyn Schmiedebergs Arch Pharmacol. 2016 Dec;389(12):1353-1366. doi: 10.1007/s00210-016-1305-8. Epub 2016 Sep 20.
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Identification of novel and robust internal control genes from Volvariella volvacea that are suitable for RT-qPCR in filamentous fungi.从草菇中鉴定适合丝状真菌 RT-qPCR 的新型稳健内参基因。
Sci Rep. 2016 Jul 12;6:29236. doi: 10.1038/srep29236.
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Regulation of GAPDH expression by treatment with the β-adrenoceptor agonist isoprenaline--is GADPH a suitable loading control in immunoblot experiments?用β-肾上腺素能受体激动剂异丙肾上腺素处理对甘油醛-3-磷酸脱氢酶（GAPDH）表达的调节——在免疫印迹实验中GAPDH是合适的上样对照吗？
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