Food and Health Programme, Institute of Food Research, Norwich Research Park, Norwich, UK.
Planta Med. 2012 Sep;78(14):1571-3. doi: 10.1055/s-0032-1315148. Epub 2012 Aug 3.
There is accumulating evidence from epidemiological and human intervention studies that quercetin-rich diets can protect against cardiovascular diseases. Quercetin glycosides are modified during metabolism, and the forms reaching the systemic circulation are glucuronidated, sulfated, and methylated. The aim of this study was to analyse the potential beneficial effects of quercetin and its conjugated metabolites on vascular function on a co-culture model of human umbilical artery smooth muscle cells and human umbilical vein endothelial cells. We observed that physiologically relevant metabolites of quercetin were able to reduce ET-1 protein and gene expression and to increase accumulation of cGMP in TNF-α-induced HUASMCs co-cultured with HUVECs. This is the first study to demonstrate an ability of quercetin and its conjugated metabolites, at physiologically achievable concentrations, to modulate vascular function in a co-culture model comprising human vascular endothelial and smooth muscle cells.
越来越多的流行病学和人体干预研究证据表明，富含槲皮素的饮食可以预防心血管疾病。槲皮素糖苷在代谢过程中会发生修饰，到达体循环的形式是葡萄糖醛酸化、硫酸化和甲基化。本研究旨在分析槲皮素及其共轭代谢物对血管功能的潜在有益影响，采用人脐动脉平滑肌细胞和人脐静脉内皮细胞共培养模型。我们观察到，槲皮素的生理相关代谢物能够降低 ET-1 蛋白和基因表达，并增加 TNF-α诱导的 HUASMCs 与 HUVECs 共培养时 cGMP 的积累。这是首次证明槲皮素及其共轭代谢物在生理可达到的浓度下能够调节包含人血管内皮和平滑肌细胞的共培养模型中的血管功能。
