Institute of Anatomy, University of Bern, Bern, Switzerland.
Cell Biochem Funct. 2013 Jan;31(1):51-9. doi: 10.1002/cbf.2859. Epub 2012 Aug 3.
To ascertain whether reactive oxygen species (ROS) contribute to training-induced adaptation of skeletal muscle, we administered ROS-scavenging antioxidants (AOX; 140 mg/l of ascorbic acid, 12 mg/l of coenzyme Q10 and 1% N-acetyl-cysteine) via drinking water to 16 C57BL/6 mice. Sixteen other mice received unadulterated tap water (CON). One cohort of both groups (CON(EXE) and AOX(EXE) ) was subjected to treadmill exercise for 4 weeks (16-26 m/min, incline of 5°-10°). The other two cohorts (CON(SED) and AOX(SED) ) remained sedentary. In skeletal muscles of the AOX(EXE) mice, GSSG and the expression levels of SOD-1 and PRDX-6 were significantly lower than those in the CON(EXE) mice after training, suggesting disturbance of ROS levels. The peak power related to the body weight and citrate synthase activity was not significantly influenced in mice receiving AOX. Supplementation with AOX significantly altered the mRNA levels of the exercise-sensitive genes HK-II, GLUT-4 and SREBF-1c and the regulator gene PGC-1alpha but not G6PDH, glycogenin, FABP-3, MCAD and CD36 in skeletal muscle. Although the administration of AOX during endurance exercise alters the expression of particular genes of the ROS metabolism, it does not influence peak power or generally shift the metabolism, but it modulates the expression of specific genes of the carbohydrate and lipid metabolism and PGC-1alpha within murine skeletal muscle.
为了确定活性氧（ROS）是否有助于骨骼肌的训练适应性，我们通过饮用水向 16 只 C57BL/6 小鼠给予 ROS 清除抗氧化剂（AOX；140mg/L 抗坏血酸、12mg/L 辅酶 Q10 和 1%N-乙酰半胱氨酸）。另外 16 只小鼠接受未稀释的自来水（CON）。两组中的一组（CON（EXE）和 AOX（EXE））进行了 4 周的跑步机运动（16-26m/min，坡度 5°-10°）。另外两组（CON（SED）和 AOX（SED））保持安静。在 AOX（EXE）小鼠的骨骼肌中，GSSG 和 SOD-1 和 PRDX-6 的表达水平在训练后明显低于 CON（EXE）小鼠，表明 ROS 水平受到干扰。在接受 AOX 的小鼠中，与体重相关的峰值功率和柠檬酸合酶活性没有受到显著影响。AOX 的补充显著改变了骨骼肌中运动敏感基因 HK-II、GLUT-4 和 SREBF-1c 以及调节基因 PGC-1alpha 的 mRNA 水平，但对 G6PDH、糖原蛋白、FABP-3、MCAD 和 CD36 没有影响。虽然在耐力运动期间给予 AOX 会改变 ROS 代谢特定基因的表达，但它不会影响峰值功率或普遍改变代谢，而是调节碳水化合物和脂质代谢以及 PGC-1alpha 在小鼠骨骼肌中的特定基因的表达。
