Department of Sport, Faculty of Physical Education, Kazimierz Wielki University in Bydgoszcz, Jana Karola Chodkiewicza 30, 85-064, Bydgoszcz, Poland.
Department of Biochemistry, Faculty of Physical Education, Gdansk University of Physical Education and Sport, Kazimierza Gorskiego 1, 80-336, Gdansk, Poland.
J Int Soc Sports Nutr. 2021 Mar 2;18(1):19. doi: 10.1186/s12970-021-00416-6.
In this study, we investigated the effects of supplementation and exercise on the expression of genes associated with inflammation like CCL2, CRP, IL1, IL6, IL10 mRNA in elderly women.
Twenty four participants divided randomly into two groups were subjected to 6 weeks of the same health training program (three times per week). SUP group (supplemented, n = 12, mean age 72.8 ± 5.26 years and mean body mass 68.1 ± 8.3 kg) received 1000 mg of Vitamin C/day during the training period, while CON group (control, n = 12, mean age 72.4 ± 5.5 years and body mass 67.7 ± 7.5 kg) received placebo.
No significant changes in IL-1, IL-6, IL-10 and CRP mRNA were observed within and between groups. However, there was a clear tendency of a decrease in IL-6 (two-way ANOVA, significant between investigated time points) and an increase in IL-10 mRNA noted in the supplemented group. A significant decrease in CCL2 mRNA was observed only in the CON group (from 2^0.2 to 2^0.1, p = 0.01).
It can be concluded, that 6 weeks of supplementation and exercise was too short to obtain significant changes in gene expression in leukocytes, but supplementation of 1000 mg vitamin C positively affected IL-6 and IL-10 expression - which are key changes in the adaptation to training. However, changes in body mass, IL1 and CCL2 were positive in CON group. It is possible that Vitamin C during 6 weeks of supplementation could have different effects on the expression of individual genes involved in the immune response.
Retrospectively registered.
本研究旨在探讨补充剂和运动对老年女性白细胞中与炎症相关基因（如 CCL2、CRP、IL1、IL6、IL10mRNA）表达的影响。
将 24 名参与者随机分为两组，均接受为期 6 周的相同健康训练计划（每周 3 次）。SUP 组（补充组，n=12，平均年龄 72.8±5.26 岁，平均体重 68.1±8.3kg）在训练期间每天补充 1000mg 维生素 C，而 CON 组（对照组，n=12，平均年龄 72.4±5.5 岁，体重 67.7±7.5kg）接受安慰剂。
两组内和组间的 IL-1、IL-6、IL-10 和 CRPmRNA 均无显著变化。然而，在补充组中观察到 IL-6（双因素方差分析，在研究的时间点之间有显著差异）有下降的明显趋势，而 IL-10mRNA 则有上升的趋势。仅在 CON 组中观察到 CCL2mRNA 显著下降（从 2^0.2 降至 2^0.1，p=0.01）。
可以得出结论，6 周的补充剂和运动时间太短，无法使白细胞中的基因表达发生显著变化，但补充 1000mg 维生素 C 可显著影响 IL-6 和 IL-10 的表达——这是对训练适应的关键变化。然而，CON 组的体重、IL1 和 CCL2 变化呈阳性。在 6 周的补充期间，维生素 C 对参与免疫反应的个别基因的表达可能有不同的影响。
回顾性注册。
