Shanghai Burns Institute, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, People's Republic of China.
J Tissue Eng Regen Med. 2014 Oct;8(10):794-800. doi: 10.1002/term.1579. Epub 2012 Aug 1.
Thorough knowledge of dermal tissue structure in three dimensions is not only a prerequisite for understanding the relationship between cells and their extracellular matrix, but also provides a basis for understanding of wound healing and scar formation for designing the ideal scaffold for skin tissue engineering. Here, we show for the first time the visualization of the three dimensional (3D) structure of dermal tissue by phase-contrast microtomography (μCT) with third-generation synchrotron radiation (SR). Compared with irregular dermal tissue (such as scar tissue), the normal dermal tissues were found to consist of a network of elliptically shaped regions containing a web of fibre bundles. The bundles, composed of fibres, were found to be orientated along specific directions, indicative of helical weaving. A regular array of dentate structure was shown on the fibres. The results showed that phase-contrast μCT with SR had a distinct advantage in accurately viewing the 3D microstructure of dermal tissues.
深入了解三维皮肤组织结构不仅是理解细胞与其细胞外基质之间关系的前提条件，也为理解伤口愈合和瘢痕形成提供了基础，从而为皮肤组织工程设计理想的支架。在这里，我们首次展示了利用第三代同步加速器辐射（SR）的相衬微断层扫描（μCT）对三维（3D）皮肤组织结构进行可视化。与不规则的皮肤组织（如瘢痕组织）相比，正常的皮肤组织由一个椭圆形区域的网络组成，其中包含纤维束的网络。纤维束由纤维组成，被发现沿特定方向取向，呈螺旋状编织。纤维上显示出规则的齿状结构排列。结果表明，SR 相衬 μCT 在准确观察皮肤组织的三维微观结构方面具有明显的优势。
