Stevens D, Alexander G, Bell A W
CSIRO, Division of Animal Production, Blacktown, NSW, Australia.
J Dev Physiol. 1990 May;13(5):277-81.
Eleven Merino sheep fetuses were supplemented with glucose by direct continuous intravenous infusion of 50% dextrose into the fetus from day 115 of gestation until spontaneous delivery. Infusion rates of 15 or 25 g/day per kg were used and equivalent volumes of saline were infused into 11 control fetuses. Infusion periods approximated 27 days in both groups. Fetal plasma glucose concentrations were significantly (P less than 0.001) elevated throughout glucose infusion and resulted in variable but consistently higher plasma insulin concentrations in the glucose than in the saline-infused fetuses. Glucose-infused fetuses were significantly heavier than controls (mean +/- SEM; 3.86 +/- 0.16 vs 3.28 +/- 0.24 kg, P less than 0.05) and body fat depots (in g/kg body wt.) were larger in glucose-infused than control fetuses (9.91 +/- 0.65 vs 6.73 +/- 0.37, P less than 0.005, for internal brown fat depots; 1.25 +/- 0.44 vs 0.27 + 0.13, P less than 0.05, for subcutaneous white adipose tissue). The results indicate that growth and lipid deposition in the sheep fetus are responsive to increased glucose supply, an effect which may be mediated through the actions of insulin. Mean gestation length was 146.60 +/- 1.45 days for controls and 144.18 +/- 1.23 days for glucose-infused animals (normal term 150 days).
从妊娠第115天起，通过直接连续静脉输注50%葡萄糖溶液至胎儿体内，直至自然分娩，对11只美利奴绵羊胎儿补充葡萄糖。使用的输注速率为每千克体重每天15克或25克，并向11只对照胎儿输注等量体积的生理盐水。两组的输注期均约为27天。在整个葡萄糖输注过程中，胎儿血浆葡萄糖浓度显著升高（P<0.001），导致输注葡萄糖的胎儿血浆胰岛素浓度高于输注生理盐水的胎儿，虽有变化但始终较高。输注葡萄糖的胎儿比对照组明显更重（平均值±标准误；3.86±0.16千克对3.28±0.24千克，P<0.05），且输注葡萄糖的胎儿体内脂肪储存（克/千克体重）比对照胎儿更大（内部棕色脂肪储存：9.91±0.65对6.73±0.37，P<0.005；皮下白色脂肪组织：1.25±0.44对0.27±0.13，P<0.05）。结果表明，绵羊胎儿的生长和脂质沉积对葡萄糖供应增加有反应，这种作用可能是通过胰岛素的作用介导的。对照组的平均妊娠期为146.60±1.45天，输注葡萄糖的动物为144.18±1.23天（正常足月为150天）。
