School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom.
Virology. 2012 Nov 10;433(1):55-63. doi: 10.1016/j.virol.2012.07.010. Epub 2012 Aug 4.
The recently released draft horse genome is incompletely characterised in terms of its repetitive element profile. This paper presents characterisation of the endogenous retrovirus (ERVs) of the horse genome based on a data-mining strategy using murine leukaemia virus proteins as queries. 978 ERV gene sequences were identified. Sequences were identified from the gamma, epsilon and betaretrovirus genera. At least one full length gammaretroviral locus was identified, though the gammaretroviral sequences are very degenerate. Using these data the RNA expression of these ERVs were derived from RNA transcriptome data from a variety of equine tissues. Unlike the well studied human and murine ERVs there do not appear to be particular phylogenetic groups of equine ERVs that are more transcriptionally active. Using this novel approach provided a more technically feasible method to characterise ERV expression than previous studies.
最近发布的马基因组在重复元件图谱方面的特征描述并不完整。本文通过使用鼠白血病病毒蛋白作为查询，基于数据挖掘策略，对马基因组中的内源性逆转录病毒（ERVs）进行了特征描述。鉴定出了 978 个 ERV 基因序列。这些序列来自于γ、ε和β逆转录病毒属。至少鉴定出了一个完整的γ逆转录病毒基因座，但γ逆转录病毒序列非常退化。利用这些数据，从各种马组织的 RNA 转录组数据中推导了这些 ERV 的 RNA 表达。与研究得很好的人类和鼠 ERV 不同，似乎没有特定的马 ERV 进化枝在转录上更活跃。与以前的研究相比，这种新方法提供了一种更具技术可行性的方法来描述 ERV 的表达。
