Fatemi S A, Mehrabani-Yeganeh H, Nejati-Javaremi A, Niknafs Sh
Department of Animal Science, University College of Agricultural and Natural Resources, University of Tehran, Karaj, Iran.
Genet Mol Res. 2012 Aug 16;11(3):2539-47. doi: 10.4238/2012.July.10.9.
Neuropeptide Y (NPY) and gonadotrophin-releasing hormone receptor (GnRHR) are two candidate genes with a wide variety of physiological functions in growth and especially in reproduction processes. We examined the association of one SNP from each of these genes with growth- and egg production-related traits in Mazandaran native chickens. Two hundred and six individuals were genotyped by PCR-RFLP. Marker-trait association analyses were performed using both breeding value and phenotypic information. The data came from 18 successive generations of selection at a Mazandaran native chicken breeding station in Iran. Data were analyzed with a univariate animal model in an ASREML procedure to estimate breeding values of the birds for these traits. Two alleles were found for both genes, A and a alleles for GnRHR, with frequencies of 0.614 and 0.386, B and b alleles for NPY, with frequencies of 0.780 and 0.221, respectively. The additive genetic effects of the GnRHR gene on egg number and egg mass were significant. Also, body weight at sexual maturity was significantly influenced by the NPY gene. We conclude that GnRHR and NPY genes are associated with egg production and growth traits, respectively.
神经肽Y（NPY）和促性腺激素释放激素受体（GnRHR）是两个具有多种生理功能的候选基因，尤其在生长过程特别是繁殖过程中。我们研究了这两个基因中各一个单核苷酸多态性（SNP）与马赞德兰本地鸡生长和产蛋相关性状的关联。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对206只个体进行基因分型。使用育种值和表型信息进行标记-性状关联分析。数据来自伊朗马赞德兰本地鸡育种站连续18代的选育。在ASREML程序中使用单变量动物模型分析数据，以估计这些性状的鸡的育种值。两个基因均发现了两个等位基因，GnRHR的A和a等位基因，频率分别为0.614和0.386，NPY的B和b等位基因，频率分别为则0.780和0.221。GnRHR基因对产蛋数和蛋重的加性遗传效应显著。此外，NPY基因对性成熟时的体重有显著影响。我们得出结论，GnRHR和NPY基因分别与产蛋和生长性状相关。
