Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
J Mol Neurosci. 2013 Mar;49(3):512-22. doi: 10.1007/s12031-012-9910-6. Epub 2012 Oct 30.
Bcl-2-associated athanogene-1 (BAG1), a co-chaperone for Hsp70/Hsc70, is a multifunctional protein, which has been shown to suppress apoptosis and enhance neuronal differentiation. However, the expression and roles of BAG1 in peripheral system lesions and repair are still unknown. In this study, we investigated the dynamic changes in BAG1 expression in an acute sciatic nerve crush model in adult rats. Western blot analysis revealed that BAG1 was expressed in normal sciatic nerves. BAG1 expression increased progressively after sciatic nerve crush, reached a peak 2 weeks post-injury, and then returned to the normal level 4 weeks post-injury. Spatially, we observed that BAG1 was mainly expressed in Schwann cells and that BAG1 expression increased in Schwann cells after injury. In vitro, we found that BAG1 expression increased during the cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation process. BAG1-specific siRNA inhibited cAMP-induced Schwann cell differentiation. In conclusion, we speculated that BAG1 was upregulated in the sciatic nerve after crush, which was associated with Schwann cell differentiation.
Bcl-2 相关抗凋亡基因 1（BAG1）是热休克蛋白 70/70（Hsp70/Hsc70）的共伴侣，是一种多功能蛋白，已被证明具有抑制细胞凋亡和促进神经元分化的作用。然而，BAG1 在周围系统损伤和修复中的表达和作用仍不清楚。在这项研究中，我们研究了 BAG1 在成年大鼠急性坐骨神经挤压模型中的表达的动态变化。Western blot 分析显示 BAG1 在正常坐骨神经中表达。坐骨神经挤压后 BAG1 的表达逐渐增加，损伤后 2 周达到峰值，然后在损伤后 4 周恢复到正常水平。空间上，我们观察到 BAG1 主要在雪旺细胞中表达，并且损伤后雪旺细胞中的 BAG1 表达增加。体外，我们发现 BAG1 的表达在环磷酸腺苷（cAMP）诱导的雪旺细胞分化过程中增加。BAG1 特异性 siRNA 抑制 cAMP 诱导的雪旺细胞分化。总之，我们推测挤压后坐骨神经中 BAG1 的上调与雪旺细胞分化有关。
