College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Anim Biotechnol. 2012;23(3):184-93. doi: 10.1080/10495398.2012.681411.
Myostatin, or growth and differentiation factor 8, is a member of the transforming growth factor-β superfamily; it functions as a negative regulator of skeletal muscle development and growth in mammals. In this study, single nucleotide polymorphisms in the 5' regulatory region and exon 1 of the myostatin gene were detected by PCR-SSCP in the Bian, Jinghai, Youxi, and Arbor Acre chickens, and the associations of the polymorphisms with reproduction traits were analyzed. Seven SNPs (A326G, C334G, C1346T, G1375A, A1473G, G1491A, and G2283A) were found in the myostatin gene. Association analysis showed that the G2283A were significantly associated with reproduction traits. Bian chickens of the GG genotype had a greater age at first egg than those of the GA and AA genotypes (P<0.01). Correspondingly, Bian chickens of the GA and AA genotypes had larger egg number at 300 days than those of the GG genotype (P<0.05 and P<0.01, respectively). Bian chickens of the AA genotype had significantly higher body weight at 300 days than those of the GG genotype (P<0.05). These results suggested that the myostatin gene may have certain effects on reproduction traits other than merely as a negative regulator of skeletal muscle development and growth in mammals previously reported.
肌肉生长抑制素，或生长分化因子 8，是转化生长因子-β超家族的成员；它作为哺乳动物骨骼肌发育和生长的负调控因子发挥作用。在这项研究中，通过 PCR-SSCP 检测了比安、静海、尤溪和阿伯克里克鸡的肌肉生长抑制素基因 5'调控区和外显子 1 中的单核苷酸多态性，并分析了这些多态性与繁殖性状的关联。在肌肉生长抑制素基因中发现了 7 个 SNP（A326G、C334G、C1346T、G1375A、A1473G、G1491A 和 G2283A）。关联分析表明，G2283A 与繁殖性状显著相关。GG 基因型的比安鸡的首次产蛋日龄大于 GA 和 AA 基因型的比安鸡（P<0.01）。相应地，GA 和 AA 基因型的比安鸡的 300 日龄产蛋数大于 GG 基因型的比安鸡（P<0.05 和 P<0.01）。AA 基因型的比安鸡的 300 日龄体重显著高于 GG 基因型的比安鸡（P<0.05）。这些结果表明，肌肉生长抑制素基因可能对繁殖性状有一定影响，而不仅仅是作为哺乳动物骨骼肌发育和生长的负调控因子。
