基于 HPLC 指纹图谱和主成分分析测定不同采收期人参中的 10 种人参皂苷。

Determination of 10 ginsenosides in Panax ginseng of different harvest times based on HPLC fingerprints and principal component analysis.

机构信息

State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China.

出版信息

Nat Prod Res. 2013;27(9):851-4. doi: 10.1080/14786419.2012.711758. Epub 2012 Aug 7.

DOI:10.1080/14786419.2012.711758

PMID:22871218

Abstract

A combinative method of HPLC fingerprinting and quantitative determination was successfully applied to monitor dynamic accumulation of ginsenosides in five-year-old Panax ginseng roots from different harvest times. The optimal chromatographic conditions were achieved on a C18 column with gradient elution using acetonitrile and 1 mmol · L(-1) KH2PO4 buffer solution at 203 nm. The result indicated that the contents of total ginsenosides showed significant variations, and a decrease tendency appeared in the growth period of the fifth year.

摘要

高效液相色谱指纹图谱与含量测定的组合方法成功地应用于监测不同收获时间的五年生人参根中人参皂苷的动态积累。在 C18 柱上，采用梯度洗脱，以乙腈和 1 mmol·L(-1) KH2PO4 缓冲溶液为流动相，在 203nm 处进行检测，得到了最佳的色谱条件。结果表明，总人参皂苷的含量变化显著，在第五年的生长期间呈下降趋势。