Chatgilialoglu Chryssostomos, Ferreri Carla, Masi Annalisa, Melchiorre Michele, Sansone Anna, Terzidis Michael A, Torreggiani Armida
Consiglio Nazionale delle Ricerche, ISOF - BioFreeRadicals, Via Piero Gobetti 101, I-40129 Bologna, Italy.
Chimia (Aarau). 2012;66(6):368-71. doi: 10.2533/chimia.2012.368.
The biological consequences of free radical production is the central subject of a very lively scientific debate, focusing on the estimation of the type and extent of damage, as well as the efficiency of the protective and repair systems. When studying free radical based chemical mechanisms, it is very important to establish biomimetic models, which allow the experiments to be performed in a simplified environment, but suitably designed to be in strict connection with cellular conditions. The biomimetic modeling approach has been coupled with physical organic chemistry methodologies and knowledge of free radical reactivity. Molecular basis of important processes have been identified, building up molecular libraries of products concerning unsaturated lipids, sulfur-containing proteins and nucleic acids, to be developed as biomarkers. Ongoing projects in our group deal with lipidomics, genomics and proteomics of free radical stress and some examples will be described.
自由基产生的生物学后果是一场非常活跃的科学辩论的核心主题，重点在于对损伤类型和程度的评估，以及保护和修复系统的效率。在研究基于自由基的化学机制时，建立仿生模型非常重要，这种模型能使实验在简化环境中进行，但经过适当设计以与细胞条件紧密相关。仿生建模方法已与物理有机化学方法及自由基反应性知识相结合。重要过程的分子基础已被确定，构建了关于不饱和脂质、含硫蛋白质和核酸的产物分子库，以开发作为生物标志物。我们团队正在进行的项目涉及自由基应激的脂质组学、基因组学和蛋白质组学，并将描述一些实例。
