Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil.
Braz J Med Biol Res. 2012 Nov;45(11):1086-94. doi: 10.1590/s0100-879x2012007500127. Epub 2012 Aug 9.
We identified different lipemic and metabolic responses after the ingestion of a standardized meal by healthy adults and related them to atherosclerotic markers. Samples from 60 normolipidemic adults were collected before and after a liquid meal (40 g fat/m² body surface) at 0, 2, 4, 6, and 8 h for measurements of lipids, free fatty acids (FFA), insulin, cholesteryl ester transfer protein (CETP), autoantibodies to epitopes of oxidized LDL (oxLDL Ab), lipolytic activities, and apolipoprotein E polymorphism. Mean carotid intima-media thickness (cIMT) was determined by Doppler ultrasound. The volunteers were classified into early (N = 39) and late (N = 31) triacylglycerol (TAG) responders to the test meal. Late responders showed lower HDL cholesterol concentration at fasting and in the TAG peak, lower insulin and higher FFA concentrations compared to early responders. Multivariate regression analyses showed that mean cIMT was associated with gender (male) and age in early responders and by cholesterol levels at the 6th hour in late responders. oxLDL Ab were explained by lipoprotein lipase and negatively by hepatic lipase and oxLDL Ab (fasting period) by CETP (negative) and FFA (positive). This study is the first to identify a postalimentary insulin resistance state, combined with a reduced CETP response exclusively among late responders, and the identification of the regulators of postalimentary atherogenicity. Further research is required to determine the metabolic mechanisms described in the different postalimentary phenotypes observed in this study, as well as in different pathological states, as currently investigated in our laboratory.
我们发现健康成年人在摄入标准化餐食后会出现不同的脂血和代谢反应，并将其与动脉粥样硬化标志物相关联。在 0、2、4、6 和 8 小时时，从 60 名血脂正常的成年人中采集样本，用于测量脂质、游离脂肪酸 (FFA)、胰岛素、胆固醇酯转移蛋白 (CETP)、氧化型 LDL 表位自身抗体 (oxLDL Ab)、脂解活性和载脂蛋白 E 多态性。通过多普勒超声测量颈动脉内膜中层厚度 (cIMT)。志愿者被分为早期（N=39）和晚期（N=31）三酰甘油（TAG）应答者。与早期应答者相比，晚期应答者在空腹和 TAG 峰值时的 HDL 胆固醇浓度较低，胰岛素浓度较低，FFA 浓度较高。多元回归分析显示，早期应答者的平均 cIMT 与性别（男性）和年龄相关，而晚期应答者的平均 cIMT 与第 6 小时的胆固醇水平相关。oxLDL Ab 与脂蛋白脂肪酶呈负相关，与肝脂肪酶呈正相关，oxLDL Ab（空腹期）与 CETP（负相关）和 FFA（正相关）呈负相关。本研究首次确定了餐后胰岛素抵抗状态，与晚期应答者中 CETP 反应降低相结合，以及确定了餐后致动脉粥样硬化性的调节因子。需要进一步研究来确定本研究中观察到的不同餐后表型以及目前在我们实验室中研究的不同病理状态中描述的代谢机制。
