Aegis Sciences Corporation, 515 Great Circle Road, Nashville, TN 37228, USA.
J Anal Toxicol. 2012 Oct;36(8):588-93. doi: 10.1093/jat/bks066. Epub 2012 Aug 7.
A number of synthetic cannabinoids such as JWH-018 and JWH-073 have been incorporated into "spice" products. Despite having labels warning against human consumption, the products are smoked for their cannabinoid-like effects and the extent of their use by athletes has not been adequately described. Urine samples collected from 5,956 athletes were analyzed by high-performance liquid chromatography-tandem mass spectrometry for the presence of JWH-018, JWH-073, and their metabolites. Metabolites of JWH-018 and/or JWH-073 were detected in 4.5% of the samples. Metabolites of JWH-018 and JWH-073, only JWH-018, and only JWH-073 were detected in 50%, 49%, and approximately 1% of positive samples, respectively. In total, JWH-018 metabolites were detected in 99% (50% + 49%) and JWH-073 metabolites were detected in approximately 50% (49% + 1%) of the positive samples. Parent JWH-018, JWH-018-2-OH-indole, and JWH-018-4-OH-indole were not detected in any of the samples. All samples in which JWH-073 metabolites were detected contained JWH-073-N-butanoic acid. Parent JWH-073 and its N-(4-OH-butyl), 4-OH-indole, 5-OH-indole, and 7-OH-indole metabolites were not detected. Given the number of synthetic cannabinoids that have been synthesized, their limited regulation, and the prevalence of JWH-018 and JWH-073 metabolites detected in the athletes, these compounds should remain a priority for anti-doping programs.
一些合成大麻素，如 JWH-018 和 JWH-073，已被掺入“香料”产品中。尽管这些产品贴有警告标签，禁止人类使用，但由于它们具有类大麻素的效果，运动员仍在使用，而且其使用程度尚未得到充分描述。通过高效液相色谱-串联质谱法分析了从 5956 名运动员采集的尿样，以检测 JWH-018、JWH-073 及其代谢物的存在。在 4.5%的样本中检测到 JWH-018 和/或 JWH-073 的代谢物。仅检测到 JWH-018 和 JWH-073 的代谢物、仅 JWH-018 代谢物和仅 JWH-073 代谢物分别占阳性样本的 50%、49%和 1%左右。总的来说，在 99%（50%+49%）的阳性样本中检测到 JWH-018 代谢物，在大约 50%（49%+1%）的阳性样本中检测到 JWH-073 代谢物。任何样本中均未检测到 JWH-018 母体、JWH-018-2-OH-吲哚和 JWH-018-4-OH-吲哚。在所有检测到 JWH-073 代谢物的样本中均含有 JWH-073-N-丁酸。JWH-073 母体及其 N-（4-OH-丁基）、4-OH-吲哚、5-OH-吲哚和 7-OH-吲哚代谢物均未检出。鉴于已经合成了如此多的合成大麻素，对其监管有限，以及在运动员中检测到的 JWH-018 和 JWH-073 代谢物的普遍性，这些化合物仍应是反兴奋剂计划的重点。
