Bureau of Forensic-Medical Expertise's, Forensic-Chemical Division, Belgorod, Russia.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 May 1;879(15-16):1126-36. doi: 10.1016/j.jchromb.2011.03.034. Epub 2011 Mar 26.
The Russian Federation prohibited the distribution of herbal mixtures with synthetic aminoalkylindoles JWH-018 and JWH-073, agonist cannabinoid receptors, on January 22, 2010. The lack or low content of their native compounds in urine requires detailed identification of their metabolites, which are excreted with urine and are present in blood. Using gas and liquid chromatography-mass spectrometry, we identified a series of metabolites in urine samples from humans and rats that were products of the following reactions: (a) mono- and dihydroxylation of the parent compounds with hydroxyl groups located at aromatic and aliphatic residues, (b) carboxylation, (c) N-dealkylation and (d) N-dealkylation and hydroxylation. The prevailing urinary metabolites in humans are monohydroxylated forms, while N-dealkylated and N-dealkyl monohydroxylated forms are found in rats. Twenty-six samples of herbal smoking mixtures with JWH-018, purchased in Russia, were analysed.
2010 年 1 月 22 日，俄罗斯联邦禁止分发含有合成氨基烷基吲哚 JWH-018 和 JWH-073（大麻素受体激动剂）的草药混合物。尿液中其天然化合物的缺乏或含量低，需要对其代谢物进行详细鉴定，这些代谢物随尿液排出，并存在于血液中。使用气相和液相色谱-质谱法，我们在人类和大鼠的尿液样本中鉴定出一系列代谢物，它们是以下反应的产物：（a）母体化合物的单羟基化和二羟基化，羟基位于芳族和脂肪族残基上，（b）羧化，（c）N-脱烷基化和（d）N-脱烷基化和羟化。在人类中，主要的尿代谢物为单羟基化形式，而在大鼠中则发现 N-脱烷基化和 N-脱烷基单羟基化形式。对在俄罗斯购买的 26 种含有 JWH-018 的草药吸烟混合物进行了分析。
