School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Analyst. 2012 Oct 7;137(19):4482-9. doi: 10.1039/c2an35535c.
In this study, a simple, fast, and reliable method to fabricate a micro free-flow electrophoresis (μFFE) device on glass is presented. The two-dimensional depth channel in the chip was easily achieved by using a photocurable monomer (NOA 81) that served as the bonding material. In such a geometrical structure (two-dimensional depth channel), the effect of fluid behavior on the separation efficiency of micro free-flow zone electrophoresis (μFFZE) was simulated. The results of numerical simulation indicate that the pressure at the inlets may play an important role in the separation performance. Under the optimum separation conditions, four FITC-labeled amino acids were well separated, indicating the validity of the performance of the chip. Since the chip was fabricated by organic polymer bonding, it was easily recyclable through a simple re-fabrication process. The reproducibility of results from these recycling re-fabrication chips was investigated. The RSD of the resolution between FITC-L-glycine and FITC-L-phenylalanine was 5.3%. Furthermore, three FITC-labeled proteins were successfully separated with the resolution of 2.2 and 5.46, respectively, by using the coating of neutral liposome.
在这项研究中，提出了一种在玻璃上制造微自由流电泳（μFFE）器件的简单、快速和可靠的方法。芯片中的二维深度通道可以通过使用光固化单体（NOA81）轻松实现，该单体用作键合材料。在这种几何结构（二维深度通道）中，模拟了流体行为对微自由流动区电泳（μFFZE）分离效率的影响。数值模拟结果表明，入口处的压力可能对分离性能起着重要作用。在最佳分离条件下，四种 FITC 标记的氨基酸得到了很好的分离，表明该芯片性能有效。由于芯片是通过有机聚合物键合制造的，因此可以通过简单的再制造过程轻松回收。研究了这些回收再制造芯片结果的重现性。FITC-L-甘氨酸和 FITC-L-苯丙氨酸之间分辨率的 RSD 为 5.3%。此外，通过使用中性脂质体的涂层，成功地分离了三种 FITC 标记的蛋白质，分辨率分别为 2.2 和 5.46。
