School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK.
Org Biomol Chem. 2012 Sep 21;10(35):7091-7. doi: 10.1039/c2ob25855b. Epub 2012 Aug 9.
A new N-benzenesulfonyl-based ionic-liquid mass spectroscopy label (I-Tag2) for covalent attachment to substrates has been prepared. I-Tag2 was used to monitor oligosaccharide elongation and serve as a purification handle. Starting from chemically synthesized I-Tag2-labelled N-acetyl glucosamine (GlcNAc) 1, I-Tag2-LacNAc (Galβ(1-4)GlcNAc) 2 and I-Tag2-Lewis(X) (Galβ(1-4)[Fucα(1-3)]GlcNAc) 3, which are oligosaccharides of biological relevance, were enzymatically prepared. The apparent kinetic parameters for the enzyme catalysed transformations with β-1,4-galactosyltransferase (β-1,4-GalT) and fucosyltransferase VI (FucT VI) were measured by LC-MS demonstrating the applicability and versatility of the new I-Tags in enzymatic transformations with glycosyltransferases.
一种新的基于 N-苯磺酰基的离子液体质谱标签（I-Tag2）已被制备用于与底物共价连接。I-Tag2 被用于监测寡糖的延伸并作为纯化的把手。从化学合成的 I-Tag2 标记的 N-乙酰葡萄糖胺（GlcNAc）1，I-Tag2-LacNAc（Galβ(1-4)GlcNAc）2 和 I-Tag2-Lewis(X)（Galβ(1-4)[Fucα(1-3)]GlcNAc）3，这是具有生物学相关性的寡糖，通过酶法制备。通过 LC-MS 测量了酶催化转化β-1,4-半乳糖基转移酶（β-1,4-GalT）和岩藻糖基转移酶 VI（FucT VI）的表观动力学参数，证明了新的 I-Tag 在糖基转移酶酶促转化中的适用性和多功能性。
