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咪唑鎓标记的糖苷作为利用人母乳中糖基转移酶活性的探针。

Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk.

作者信息

Sittel I, Galan M C

机构信息

School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.

出版信息

Org Biomol Chem. 2017 May 3;15(17):3575-3579. doi: 10.1039/c7ob00550d.

DOI:10.1039/c7ob00550d
PMID:28401975
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5708356/
Abstract

Imidazolium-labeled (ITag-) glycosides are used to harness the glycosyltransferase activity directly from human breast milk. The covalently attached ionic labels provide a bifunctional chemical handle that is used to monitor reaction progress by MS, as well as aid in product purification from complex mixtures. The technology is exemplified in the synthesis of biologically relevant oligosaccharide analogs, LacNAc-ITag, ITag-Lewis and ITag-Lewis, in a matter of days from human breast milk without having to isolate specific enzymes.

摘要

咪唑鎓标记的（ITag-）糖苷用于直接利用人母乳中的糖基转移酶活性。共价连接的离子标签提供了一种双功能化学手柄，可用于通过质谱监测反应进程，并有助于从复杂混合物中纯化产物。该技术在从人母乳中在几天内合成生物学相关的寡糖类似物LacNAc-ITag、ITag-岩藻糖和ITag-唾液酸的过程中得到了体现，而无需分离特定的酶。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/92dedb55a956/c7ob00550d-s4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/21d8c3b4f57f/c7ob00550d-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/72bb69e12310/c7ob00550d-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/6bb119fd4e5f/c7ob00550d-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/dedfff093a29/c7ob00550d-s3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/92dedb55a956/c7ob00550d-s4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/21d8c3b4f57f/c7ob00550d-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/72bb69e12310/c7ob00550d-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/6bb119fd4e5f/c7ob00550d-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/dedfff093a29/c7ob00550d-s3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f82/5708356/92dedb55a956/c7ob00550d-s4.jpg

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