Kahn A, Brachet E
Biochim Biophys Acta. 1979 Dec 3;588(2):219-31. doi: 10.1016/0304-4165(79)90205-8.
In order to study the mechanisms whereby mediators of inflammation exert their exudative effects, we used isolated rat mesentery placed as a separation membrane between the two compartments of a diffusion cell. In this experimental arrangement, the permeability coefficient of albumin (PA) can be easily computed from the equilibration rate of 125I-labelled albumin added to one compartment. Histamine, bradykinin, serotonin and prostaglandins A1, A2, E1, E2, F1 alpha and F2 alpha all increased PA to some extent, the maximal values being approx. +60%. Dibutyryl-cyclic AMP, theophylline and isoproterenol also increased PA, thus suggesting involvement of cyclic AMP. Direct measurements of this nucleotide confirmed this hypothesis; furthermore, a linear relation between cyclic AMP levels and PA could be demonstrated. In contrast, cyclic GMP is probably not involved in the control of PA. Calcium-depleted tissues had a low PA (approx. 40% below controls), and did not respond to exogenous prostaglandin E1. These results suggest that transmesenteric passage of albumin is at least partly controlled by cyclic AMP and intracellular Ca2+ levels.
为了研究炎症介质发挥其渗出作用的机制，我们使用分离的大鼠肠系膜作为扩散池两个隔室之间的分隔膜。在这种实验设置中，白蛋白的通透系数（PA）可以根据添加到一个隔室中的125I标记白蛋白的平衡速率轻松计算得出。组胺、缓激肽、血清素以及前列腺素A1、A2、E1、E2、F1α和F2α均在一定程度上增加了PA，最大值约为+60%。二丁酰环磷腺苷、茶碱和异丙肾上腺素也增加了PA，因此提示环磷腺苷参与其中。对这种核苷酸的直接测量证实了这一假设；此外，还可以证明环磷腺苷水平与PA之间存在线性关系。相比之下，环鸟苷酸可能不参与PA的调控。缺钙组织的PA较低（比对照组低约40%），并且对外源性前列腺素E1无反应。这些结果表明，白蛋白的跨肠系膜转运至少部分受环磷腺苷和细胞内钙离子水平的控制。
