Kopczyński Z, Dryl-Rydzyńska T, Baczyk K
Zakładu Analityki Medycznej Katedry Onkologii, Karola Marcinkowskiego w Poznaniu.
Pol Tyg Lek. 1990;45(27-28):565-7.
An effect of methylguanidine and guanidinosuccinic acid on pyruvate kinase activity in human red cells was determined in vitro following a 3-hour incubation at 37 degrees C. The obtained results have shown that methylguanidine in the concentration of 1.8 x 10(-5) M/l inhibits pyruvate kinase activity by 20.8%. Pyruvate kinase activity was statistically significantly inhibited on addition of methylguanidine in the concentration of 5.4 x 10(-5) M/l whereas higher concentrations have no such an effect Guanidinesuccinic acid exerted similar but weaker effect on the activity of pyruvate kinase in human red cells. Mixture of methylguanidine (5.4 x 10(-5) m/l) and guanidinesuccinic acid (2.8 x 10(-5) M/l) does not affect pyruvate kinase activity in normal human red cells under identical experimental conditions.
在37摄氏度下孵育3小时后，于体外测定了甲基胍和胍基琥珀酸对人红细胞中丙酮酸激酶活性的影响。所得结果表明，浓度为1.8×10⁻⁵M/L的甲基胍可使丙酮酸激酶活性降低20.8%。加入浓度为5.4×10⁻⁵M/L的甲基胍后，丙酮酸激酶活性受到统计学显著抑制，而更高浓度则无此作用。胍基琥珀酸对人红细胞中丙酮酸激酶的活性也有类似但较弱的影响。在相同实验条件下，甲基胍（5.4×10⁻⁵m/L）和胍基琥珀酸（2.8×10⁻⁵M/L）的混合物不影响正常人红细胞中的丙酮酸激酶活性。
