外周血和腹水淋巴细胞氧化 DNA 损伤与癌症患者。
Oxidative dna damage of lymphocytes in peripheral blood and ascites in cancer patients.
机构信息
Department of Experimental Center, Yidu Central Hospital of Weifang City, Weifang, PR China.
出版信息
Curr Oncol. 2012 Jul;19(Suppl 2):eS10-4. doi: 10.3747/co.19.1136.
BACKGROUND
Patients with malignant ascites (ma) usually experience poor quality of life, and treatment of this symptom remains a challenge. Oxidative stress, which can cause oxidative damage to dna, plays a pivotal role in carcinogenesis; however, the relationship between oxidative stress and dna damage to tumour-associated lymphocytes (tals) in ma is unclear.
METHODS
We measured the total antioxidant capacity (tac) of plasma and ma supernatant in 31 cancer patients with ma, and we used a comet assay to assess dna damage to both peripheral blood mononuclear cells (pbmcs) and tals. Measurements in age- and sex-matched healthy volunteers were used as controls.
RESULTS
The tac of plasma was remarkably lower in cancer patients (9.73 ± 1.96 U/mL) than in healthy control subjects (11.31 ± 1.50 U/mL, p < 0.001). The tac of ma supernatant (6.34 ± 1.57 U/mL) was significantly lower than that of plasma in cancer patients (7.42 ± 1.36 U/mL, p < 0.001). The comet percentage of pbmcs was higher in cancer patients (17.26% ± 6.04%) than in healthy control subjects (9.44% ± 4.47%, p < 0.01). In cancer patients, the comet percentage of tals (36.14% ± 17.85%) was significantly higher than that of pbmcs (17.26% ± 6.04%, p < 0.001). In cancer patients with ma, negative correlations were observed between plasma tac and dna damage to pbmcs (r = -0.505, p = 0.004) and between the tac of ma supernatant and the comet percentage of tals (r = -0.588, p = 0.001).
CONCLUSIONS
Results indicate the presence of significant oxidative damage to the dna of lymphocytes in peripheral blood and ascites from patients with ma, being especially higher in the cells from ascites. The lower tac of ma supernatant may be related to a higher degree of dna damage to tals. The present study suggests that an oxidant-antioxidant imbalance may be one of the mechanisms leading to the dna damage detected in peripheral blood and local tals in patients with ma, which may provide a novel approach to the treatment of ma.
背景
患有恶性腹水（MA）的患者通常生活质量较差，而这种症状的治疗仍然是一个挑战。氧化应激可导致 DNA 氧化损伤，在致癌作用中起关键作用；然而，MA 中肿瘤相关淋巴细胞（TALs）的氧化应激与 DNA 损伤之间的关系尚不清楚。
方法
我们测量了 31 例 MA 癌症患者血浆和 MA 上清液的总抗氧化能力（TAC），并使用彗星试验评估外周血单核细胞（PBMCs）和 TALs 的 DNA 损伤。将年龄和性别匹配的健康志愿者的测量值用作对照。
结果
癌症患者的血浆 TAC 显着低于健康对照组（9.73 ± 1.96 U/mL）（p <0.001）。癌症患者 MA 上清液的 TAC（6.34 ± 1.57 U/mL）明显低于血浆（7.42 ± 1.36 U/mL，p <0.001）。癌症患者 PBMCs 的彗星百分比（17.26% ± 6.04%）高于健康对照组（9.44% ± 4.47%，p <0.01）。在癌症患者中，TALs 的彗星百分比（36.14% ± 17.85%）明显高于 PBMCs 的彗星百分比（17.26% ± 6.04%，p <0.001）。在 MA 癌症患者中，血浆 TAC 与 PBMCs 的 DNA 损伤之间呈负相关（r = -0.505，p = 0.004），MA 上清液的 TAC 与 TALs 的彗星百分比之间呈负相关（r = -0.588，p = 0.001）。
结论
结果表明，MA 患者外周血和腹水淋巴细胞的 DNA 存在明显的氧化损伤，腹水细胞中的损伤更为严重。MA 上清液的 TAC 较低可能与 TALs 的 DNA 损伤程度较高有关。本研究表明，氧化还原失衡可能是导致 MA 患者外周血和局部 TALs 检测到的 DNA 损伤的机制之一，为 MA 的治疗提供了新的方法。
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