Aslan Mehmet, Nazligul Yaşar, Bolukbas Cengiz, Bolukbas Fusun F, Horoz Mehmet, Dulger Ahmet C, Erdur Fatih M, Celik Hakim, Kocyigit Abdurrahim
Yuzuncu Yil University, Medical Faculty, Department of Internal Medicine, Van, Turkey.
Pol Arch Med Wewn. 2011 Jul-Aug;121(7-8):223-9.
Ulcerative colitis (UC) is a fairly common chronic inflammatory disorder. Chronic inflammation may contribute to the risk of colorectal cancer through the accumulation of specific products resulting from DNA damage. Previous studies reported that DNA damage and oxidative stress play a significant role in the pathophysiology of UC, but the results are inconsistent.
In the present study, we investigated peripheral DNA damage and oxidative stress in patients with UC.
The study included 20 patients with UC and 20 controls. Peripheral lymphocyte DNA damage was measured using the alkaline comet assay. Plasma total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined.
DNA damage levels, TOS, and OSI were significantly higher in patients with UC than in controls (P <0.001 for all parameters), while TAC was significantly lower (P <0.001). DNA damage was significantly correlated with TOS, TAC, and OSI (r = 0.604, P <0.001; r = -0.593, P <0.001; and r = 0.716, P <0.001, respectively). Moreover, TAC levels were significantly correlated with TOS and OSI (r = 0.604, P <0.001; r = -0.399, P <0.05; and r = -0.513, P <0.05, respectively).
Our results show that increased peripheral DNA damage and oxidative stress seem to be associated with decreased antioxidant levels and thus may in part contribute to the development of colorectal cancer associated with UC.
溃疡性结肠炎（UC）是一种相当常见的慢性炎症性疾病。慢性炎症可能通过DNA损伤产生的特定产物积累，增加患结直肠癌的风险。先前的研究报道，DNA损伤和氧化应激在UC的病理生理学中起重要作用，但结果并不一致。
在本研究中，我们调查了UC患者外周血DNA损伤和氧化应激情况。
本研究纳入20例UC患者和20例对照。采用碱性彗星试验检测外周淋巴细胞DNA损伤。测定血浆总抗氧化能力（TAC）、总氧化状态（TOS）和氧化应激指数（OSI）。
UC患者的DNA损伤水平、TOS和OSI显著高于对照组（所有参数P<0.001），而TAC显著降低（P<0.001）。DNA损伤与TOS、TAC和OSI显著相关（r分别为0.604，P<0.001；r=-0.593，P<0.001；r=0.716，P<0.001）。此外，TAC水平与TOS和OSI显著相关（r分别为0.604，P<0.001；r=-0.399，P<0.05；r=-0.513，P<0.05）。
我们的结果表明，外周血DNA损伤增加和氧化应激似乎与抗氧化水平降低有关，因此可能在一定程度上促成了与UC相关的结直肠癌的发生。
