HBV 患者的受精卵中存在 HBV mRNA，证实了 HBV 通过卵子进行垂直传播。
The presence of HBV mRNA in the fertilized in vitro embryo of HBV patients confirms vertical transmission of HBV via the ovum.
机构信息
Departments of Infectious Disease and Gynecology and Obstetrics, The First Hospital of Xi'an Jiaotong University, Xi'an, China.
出版信息
Epidemiol Infect. 2013 May;141(5):926-30. doi: 10.1017/S0950268812001690. Epub 2012 Aug 9.
Abstract
This study aimed to confirm that vertical transmission of hepatitis B virus (HBV) can occur via the infected ovum. Specimens studied were obtained from discarded test-tube embryos from mothers with chronic HBV infection who had received in vitro fertilization treatment. Single-cell reverse transcriptase-polymerase chain reaction was used to detect HBV mRNA in the embryos. HBV mRNA was detected in the cleavage embryos of patients with chronic HBV infection, with a detection rate of 13.2% (5/38). The level of serum HBV DNA was not related to the HBV mRNA positivity rates in embryos. In this study, HBV mRNA was detected in test-tube embryos from HBV-infected mothers who had received in vitro fertilization treatment. This confirms the theory of vertical transmission of HBV via the ovum, thereby providing an important theoretical basis for further study on the mechanism of HBV vertical transmission, influencing factors and blocking measures.
摘要
本研究旨在证实乙型肝炎病毒（HBV）可通过受感染的卵子进行垂直传播。研究标本取自接受体外受精治疗的慢性 HBV 感染母亲废弃的试管胚胎。采用单细胞逆转录-聚合酶链反应检测胚胎中的 HBV mRNA。在慢性 HBV 感染患者的分裂胚胎中检测到 HBV mRNA，阳性率为 13.2%（5/38）。血清 HBV DNA 水平与胚胎中 HBV mRNA 阳性率无关。本研究在接受体外受精治疗的 HBV 感染母亲的试管胚胎中检测到 HBV mRNA，证实了 HBV 通过卵子垂直传播的理论，为进一步研究 HBV 垂直传播的机制、影响因素和阻断措施提供了重要的理论依据。
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