Sato Hiroki, Saburi Wataru, Ojima Teruyo, Taguchi Hidenori, Mori Haruhide, Matsui Hirokazu
Research Faculty of Agriculture, Hokkaido University, N-9, W-9, Sapporo 060-8589, Japan.
Biosci Biotechnol Biochem. 2012;76(8):1584-7. doi: 10.1271/bbb.120284. Epub 2012 Aug 7.
Cellobiose 2-epimerase (CE) efficiently forms epilactose which has several beneficial biological functions. A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column. Lactose (100 g/L) was supplied to the reactor, kept at 50 °C at a space velocity of 8 h(-1). The epilactose concentration of the resulting eluate was 30 g/L, and this was maintained for 13 d.
纤维二糖2-差向异构酶（CE）能高效地形成具有多种有益生物学功能的表乳糖。将来自海栖热袍菌的一种耐热CE固定在杜里特A568上并填充到柱中。向反应器中供应乳糖（100 g/L），在50℃下以8 h⁻¹的空速保持。所得洗脱液中的表乳糖浓度为30 g/L，并且这种情况维持了13天。
