Bródka Karolina, Sowiak Małgorzata, Kozajda Anna, Cyprowski Marcin, Irena Szadkowska-Stańczyk
Department of Environmental Health Hazards, Nofer Institute of Occupational Medicine, Łódź, Poland.
Med Pr. 2012;63(3):303-15.
Indoor air is contaminated with microorganisms coming from both the atmospheric air and sources present in premises. The aim of this study was to analyze the concentrations of biological agents in office buildings, dependending on ventilation/air conditioning system and season.
The study covered office buildings (different in the system of ventila-tion/air conditioning). Air samples for assessing the levels of inhalable dust, endotoxins and (1-->3)-beta-D-glucans, were taken at the selected stationary points of each building during summer and winter. The air was sampled for 6 h, using portable sets consisting of the GilAir 5 pump and the head filled with a filter of fiber glass. The samples for the presence of airborne bacteria and fungi were collected twice during the day using the impaction method.
Average concentrations of inhalable dust, bacteria, fungi, endotoxins and (1-->3)-beta-D-glucans in office premises were 0.09 mg/m3, 6.00 x 10(2) cfu/m3, 4.59 x 10(1) cfu/m3, 0.42 ng/m3 and 3.91 ng/m3, respectively. Higher concentrations of the investigated agents were found in summer. In premises with air conditioning concentrations of airborne fungi, (1-->3)-beta-D-glucans and inhalable dust were significantly lower in winter. In summer the trend was reverse except for (1-->3)-beta-D-glucans.
Concentrations of biological agents were affected by the season and the presence of air conditioning. Concentrations of inhalable dust, bacteria, fungi, endotoxins and (1-->3)-beta-D-glucans, observed inside the office buildings, were significantly higher in summer than in winter. The presence of the air conditioning system modified in various ways the levels of biological agents. Its influence was greater on the concentration of fungi and (1-->3)-beta-D-glucans than on that of bacteria and endotoxins.
室内空气受到来自大气和室内源的微生物污染。本研究的目的是分析办公楼中生物因子的浓度，其取决于通风/空调系统和季节。
该研究涵盖了（通风/空调系统不同的）办公楼。在夏季和冬季，于每栋建筑选定的固定点采集空气样本，以评估可吸入粉尘、内毒素和（1→3）-β-D-葡聚糖的水平。使用由GilAir 5泵和装有玻璃纤维过滤器的采样头组成的便携式设备采集6小时的空气样本。采用撞击法，在白天对空气中细菌和真菌的存在情况进行两次采样。
办公楼内可吸入粉尘、细菌、真菌、内毒素和（1→3）-β-D-葡聚糖的平均浓度分别为0.09毫克/立方米、6.00×10² 菌落形成单位/立方米、4.59×10¹ 菌落形成单位/立方米、0.42纳克/立方米和3.91纳克/立方米。夏季发现所研究因子的浓度较高。在有空调的场所，冬季空气中真菌、（1→3）-β-D-葡聚糖和可吸入粉尘的浓度显著较低。除（1→3）-β-D-葡聚糖外，夏季趋势相反。
生物因子的浓度受季节和空调的影响。办公楼内观察到的可吸入粉尘、细菌、真菌、内毒素和（1→3）-β-D-葡聚糖的浓度夏季显著高于冬季。空调系统的存在以多种方式改变了生物因子的水平。其对真菌和（1→3）-β-D-葡聚糖浓度的影响大于对细菌和内毒素浓度的影响。
