Rogala Ewelina, Nowicka Aldona, Bednarek Wiesława, Barczyński Bartłomiej, Wertel Iwona, Zakrzewski Maciej, Kotarski Jan
I Katedra i Klinika Ginekologii Onkologicznej i Ginekologii Uniwersytetu Medycznego w Lublinie, Polska.
Ginekol Pol. 2012 Jun;83(6):424-8.
Knowledge of the role of interleukin 17 (IL-17) has led to the identification of new subpopulation of T helper lymphocytes--Th17 and T cytotoxic lymphocytes secreting IL-17 (Tc17). An increasing amount of attention is paid to their role in anti-tumor immunity.
The aim of this study was to evaluate the percentage of peripheral blood, peritoneal fluid and cancer tissue CD4+ and CD8+ T lymphocytes producing IL-17 in patients with ovarian cancer
Forty patients operated due to advanced ovarian carcinoma and twenty-four patients with functional follicle ovarian cysts were recruited. Peripheral blood, peritoneal fluid and cancer tissue mononuclear cells from ovarian cancer patients were stimulated for 4 hours ex vivo with phorbol myristate acetate (PMA) (50 ng/ ml) and ionomycin (1 microg/ml). The percentage of CD4+ and CD8+ T cells producing IL-17 was measured using flow cytometry.
CD4+ and CD8+ T lymphocytes producing IL-17 were detected in the peripheral blood, peritoneal fluid and cancer tissue of ovarian cancer patients. The percentage of CD4+ T cells producing IL-17 was higher in the peripheral blood, peritoneal fluid and cancer tissue when compared to CD8+/IL 17+ T cells. The percentage of CD4+/ IL-17+ was significantly higher in cancer tissue compared to T cells derived form peripheral blood. There was no difference in the percentage of CD4+/IL-17 + T cells between peripheral blood and peritoneal fluid and peritoneal fluid and cancer tissue of ovarian cancer patients. There was no difference in the percentage of CD8+/IL-17 + T lymphocytes in the peripheral blood, peritoneal fluid and cancer tissue in patients suffering from ovarian cancer.
Increased percentage of CD4+/IL-17+ and CD8+/IL-17+ T cells in cancer tissue indicates that these cells are accumulated in ovarian cancer microenvironment.
对白介素17（IL-17）作用的了解促使人们识别出了辅助性T淋巴细胞的新亚群——Th17以及分泌IL-17的细胞毒性T淋巴细胞（Tc17）。它们在抗肿瘤免疫中的作用受到越来越多的关注。
本研究旨在评估卵巢癌患者外周血、腹腔积液及癌组织中产生IL-17的CD4+和CD8+T淋巴细胞的百分比。
招募了40例因晚期卵巢癌接受手术的患者以及24例功能性卵泡卵巢囊肿患者。将卵巢癌患者的外周血、腹腔积液及癌组织单核细胞在体外使用佛波酯（PMA）（50 ng/ml）和离子霉素（1 μg/ml）刺激4小时。使用流式细胞术检测产生IL-17的CD4+和CD8+T细胞的百分比。
在卵巢癌患者的外周血、腹腔积液及癌组织中检测到了产生IL-17的CD4+和CD8+T淋巴细胞。与CD8+/IL 17+T细胞相比，外周血、腹腔积液及癌组织中产生IL-17的CD4+T细胞百分比更高。与外周血来源的T细胞相比，癌组织中CD4+/ IL-17+的百分比显著更高。卵巢癌患者外周血与腹腔积液以及腹腔积液与癌组织之间CD4+/IL-17 +T细胞的百分比没有差异。卵巢癌患者外周血、腹腔积液及癌组织中CD8+/IL-17 +T淋巴细胞的百分比没有差异。
癌组织中CD4+/IL-17+和CD8+/IL-17+T细胞百分比增加表明这些细胞在卵巢癌微环境中积聚。
