Köpf-Maier P
Institut für Anatomie, Freie Universität Berlin.
Acta Anat (Basel). 1990;139(2):164-72. doi: 10.1159/000146994.
In the present study, the distribution of phosphorus was analyzed within lysosomes of hepatocytes, Kupffer cells and mast cells in untreated liver tissue of mice by the use of electron-spectroscopic imaging, a new electron-microscopical method which combines microanalysis with ultrastructural representation and allows to image the spatial distribution of light elements such as O, N or P in cells and tissues. The obtained analytical data unfolded marked differences regarding the phosphorus content of lysosomes within hepatocytes. Whereas peribiliary bodies always included phosphorus highly condensed in a coarse-granular distribution, the lysosomes apart from biliary capillaries contained different amounts of phosphorus, varying from high concentration to moderate and negligible amounts appearing as dust-like powder in the lysosomal interior. In contrast, the lysosomes of Kupffer cells always comprised phosphorus in high density and similar concentration as it is found in cytoplasmic ribosomes. These results confirm a pronounced and unexpected heterogeneity regarding the elemental composition of lysosomes in hepatocytes and Kupffer cells. It may be assumed that this heterogeneity is not accidental but based on differences in the functional state of lysosomes and the activity of their hydrolytic enzymes.
在本研究中，利用电子能谱成像技术分析了小鼠未处理肝脏组织中肝细胞、库普弗细胞和肥大细胞溶酶体内磷的分布情况。电子能谱成像是一种新的电子显微镜方法，它将微观分析与超微结构表征相结合，能够对细胞和组织中诸如氧、氮或磷等轻元素的空间分布进行成像。所获得的分析数据揭示了肝细胞内溶酶体磷含量存在显著差异。胆小管周围小体总是含有高度浓缩的磷，呈粗颗粒分布，而胆管毛细血管外的溶酶体含有不同量的磷，从高浓度到中等浓度，甚至在溶酶体内部呈尘埃状粉末的可忽略不计的量。相比之下，库普弗细胞的溶酶体总是含有高密度且浓度与细胞质核糖体中相似的磷。这些结果证实了肝细胞和库普弗细胞溶酶体的元素组成存在明显且出乎意料的异质性。可以推测这种异质性并非偶然，而是基于溶酶体功能状态及其水解酶活性的差异。
