Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Mycoses. 2013 Mar;56(2):150-6. doi: 10.1111/j.1439-0507.2012.02225.x. Epub 2012 Aug 12.
Aureobasidin A (AbA) is a cyclic depsipeptide antifungal compound that inhibits a wide range of pathogenic fungi. In this study, the in vitro susceptibility of 92 clinical isolates of various Candida species against AbA was assessed by determining the planktonic and biofilm MICs of the isolates. The MIC(50) and MIC(90) of the planktonic Candida yeast were 1 and 1 μg ml(-1), respectively, whereas the biofilm MIC(50) and MIC(90) of the isolates were 8 and ≥64 μg ml(-1) respectively. This study demonstrates AbA inhibition on filamentation and biofilm development of C. albicans. The production of short hyphae and a lack of filamentation might have impaired biofilm development of AbA-treated cells. The AbA resistance of mature Candidia biofilms (24 h adherent population) was demonstrated in this study.
金褐霉素 A（AbA）是一种环状脂肽类抗真菌化合物，可抑制多种致病性真菌。在这项研究中，通过测定分离株的浮游和生物膜 MIC，评估了 92 株临床分离的各种念珠菌对 AbA 的体外药敏性。浮游念珠菌酵母的 MIC（50）和 MIC（90）分别为 1 和 1 μg ml（-1），而分离株的生物膜 MIC（50）和 MIC（90）分别为 8 和≥64 μg ml（-1）。本研究表明 AbA 可抑制白念珠菌的丝状形成和生物膜的发展。AbA 处理细胞的短菌丝体的产生和丝状形成的缺乏可能损害了生物膜的发展。本研究证明了成熟念珠菌生物膜（24 小时附着群体）对 AbA 的耐药性。
