St Paul Michael, Paolucci Sarah, Read Leah R, Sharif Shayan
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Vet Immunol Immunopathol. 2012 Oct 15;149(3-4):237-44. doi: 10.1016/j.vetimm.2012.07.008. Epub 2012 Jul 22.
Toll-like receptors (TLRs) are an evolutionarily conserved group of pattern recognition receptors that play an important role in mediating host-responses to pathogens. Several TLRs have been identified in chickens and their expression has been detected in many immune cell subsets including in B cells. However, the mechanisms through which TLRs modulate B cell responses have not been well characterized in chickens. The aim of the present study was to elucidate the responses mounted by cells of the bursa of Fabricius to treatment with the TLR 3, 4 and 21 ligands, poly I:C, lipopolysaccharide (LPS) and CpG oligodeoxynucleotides (ODN), respectively. The relative expression of several immune system genes was quantified at 1, 3, 8 and 18 h post-treatment. The results show that all three ligands induced the up-regulation of interferon (IFN)-γ and interleukin (IL)-10 transcripts and promoted the up-regulation of transcripts associated with antigen presentation, namely CD80 and major histocompatibility complex (MHC) class II. Furthermore, the results indicated that LPS and poly I:C induced the greatest IFN-γ and IL-10 responses, respectively, while CpG ODN was the most efficacious inducer of CD80 and MHC-II expression. Future studies may be aimed at elucidating the mechanisms of TLR-mediated activation of chicken B cells. These mechanisms may be then exploited for the development of adjuvants with enhanced ability to stimulate B cell responses.
Toll样受体（TLRs）是一组进化上保守的模式识别受体，在介导宿主对病原体的反应中起重要作用。鸡体内已鉴定出几种TLRs，并且在包括B细胞在内的许多免疫细胞亚群中都检测到了它们的表达。然而，TLRs调节鸡B细胞反应的机制尚未得到充分表征。本研究的目的是阐明法氏囊细胞对分别用TLR 3、4和21配体，即聚肌胞苷酸（poly I:C）、脂多糖（LPS）和CpG寡脱氧核苷酸（ODN）处理后的反应。在处理后1、3、8和18小时对几种免疫系统基因的相对表达进行了定量。结果表明，所有三种配体均诱导干扰素（IFN）-γ和白细胞介素（IL）-10转录本上调，并促进与抗原呈递相关的转录本上调，即CD80和主要组织相容性复合体（MHC）II类。此外，结果表明LPS和聚肌胞苷酸分别诱导了最大的IFN-γ和IL-10反应，而CpG ODN是CD80和MHC-II表达的最有效诱导剂。未来的研究可能旨在阐明TLR介导的鸡B细胞活化机制。然后可以利用这些机制开发具有增强刺激B细胞反应能力的佐剂。
