Department of Anatomy and Cell Biology, Stark Neuroscience Research Institute, Indiana Spinal Cord and Brain Injury Research Group, Indiana University School of Medicine, 980 W. Walnut Street, Indianapolis, IN 46202, USA.
J Neurosci Methods. 2012 Sep 30;210(2):119-24. doi: 10.1016/j.jneumeth.2012.07.019. Epub 2012 Jul 31.
We introduce a method that uses optogenetic stimulation to evoke field potentials in brain slices prepared from transgenic mice expressing channelrhodopsin-2-YFP. Cortical slices in a recording chamber were stimulated with a 473 nm blue laser via either a laser scanning photostimulation setup or by direct guidance of a fiber optic. Field potentials evoked by either of the two optogenetic stimulation methods had stable amplitude, consistent waveform, and similar components as events evoked with a conventional stimulating electrode. The amplitude of evoked excitatory postsynaptic potentials increased with increasing laser intensity or pulse duration. We further demonstrated that optogenetic stimulation can be used for the induction and monitoring of long-term depression. We conclude that this technique allows for efficient and reliable activation of field potentials in brain slice preparation, and will be useful for studying short and long term synaptic plasticity.
我们介绍了一种方法，该方法使用光遗传学刺激来诱发来自表达通道视紫红质-2-YFP 的转基因小鼠的脑片上的场电位。在记录室中的皮质片通过激光扫描光刺激设置或通过光纤的直接引导用 473nm 蓝光激光进行刺激。通过这两种光遗传学刺激方法之一诱发的场电位具有稳定的幅度、一致的波形和与用传统刺激电极诱发的事件相似的成分。诱发的兴奋性突触后电位的幅度随着激光强度或脉冲持续时间的增加而增加。我们进一步证明，光遗传学刺激可用于诱导和监测长时程抑制。我们的结论是，该技术允许在脑片制备中有效地和可靠地激活场电位，并且将有助于研究短期和长期突触可塑性。
