Alencar Nara L M, Innecco Renato, Gomes-Filho Enéas, Gallão Maria Izabel, Alvarez-Pizarro Juan C, Prisco José T, Oliveira Alexandre B De
Departamento de Fitotecnia, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
An Acad Bras Cienc. 2012 Sep;84(3):823-32. doi: 10.1590/s0001-37652012000300024.
Cereus jamacaru, a Cactaceae found throughout northeast Brazil, is widely used as cattle food and as an ornamental and medicinal plant. However, there has been little information about the physiological and biochemical aspects involved in its germination. The aim of this study was to investigate its reserve mobilization during germination and early seedling growth. For this, C. jamacaru seeds were germinated in a growth chamber and collected at 0, 2, 4, 5, 6, 8 and 12 days after imbibition for morphological and biochemical analyses. Dry seeds had wrinkled seed coats and large, curved embryos. Lipids were the most abundant reserve, comprising approximately 55% and 65% of the dry mass for cotyledons and the hypocotylradicle axis, respectively. Soluble sugars and starch were the minor reserves, corresponding to approximately 2.2% of the cotyledons' dry mass, although their levels showed significant changes during germination. Soluble proteins corresponded to 40% of the cotyledons' dry mass, which was reduced by 81% at the final period of germination compared to dry seeds. C. jamacaru seed can be classified as an oil seed due to its high lipid content. Moreover, lipids were the main reserve mobilized during germination because their levels were strongly reduced after seed germination, while proteins were the second most utilized reserve in this process.
仙人掌科植物刺梨分布于巴西东北部各地，被广泛用作牛的饲料以及观赏和药用植物。然而，关于其萌发过程中涉及的生理和生化方面的信息却很少。本研究的目的是调查其在萌发和幼苗早期生长过程中的储备物质动员情况。为此，将刺梨种子在生长室中萌发，并在吸胀后0、2、4、5、6、8和12天收集，进行形态学和生化分析。干燥种子的种皮有皱纹，胚大且弯曲。脂质是最丰富的储备物质，分别占子叶和下胚轴-胚根轴干重的约55%和65%。可溶性糖和淀粉是次要储备物质，约占子叶干重的2.2%，尽管其含量在萌发过程中发生了显著变化。可溶性蛋白质占子叶干重的40%，与干燥种子相比，在萌发末期减少了81%。由于刺梨种子的脂质含量高，可被归类为油籽。此外，脂质是萌发过程中主要被动员的储备物质，因为种子萌发后其含量大幅降低，而蛋白质是此过程中第二大被利用的储备物质。
