Departments of Rheumatology and Rehabilitation, Faculty of Medicine, EL-Minia University, EL-Minia, Egypt.
Rheumatol Int. 2013 Apr;33(4):1021-5. doi: 10.1007/s00296-012-2464-7. Epub 2012 Aug 12.
Primary Sjögren's syndrome (PSS) is associated with increased risk of lymphoproliferative malignancy, and B-cell non-Hodgkin lymphoma (B-NHL) is the most frequent type. To evaluate CD4+ T lymphocytes distributions in patients with (PSS) and the association of CD4+ T lymphocytopenia with the development of (B-NHL), this study included 8 (PSS) patients associated with B-NHL (group I), 50 (pSS) patients without B-NHL (group II), and 30 healthy volunteers who served as controls. The frequency of circulating CD4+ and CD8+ T lymphocytes distributions and CD4+/CD8+ T cell ratio was assessed using flow cytometry coulter EPICS-XL and compared between patients groups and controls. There was statistically significant CD4+ T lymphocytopenia in (PSS) patients with B-NHL than those without lymphoma and controls (P = 0.001). Moreover, a significant low CD4+/CD8+ T cell ratio 0.8 in group I than group II and controls (P = 0.001) was found. Significant positive correlations of CD4+ T lymphocytopenia with other risk factors (parotid swelling, vasculitis, rheumatoid factors, low complement, cryoglobulinemia) were detected. CD4+ T lymphocytopenia is associated with B-NHL developed in patients with PSS and can be considered as an important predictor of lymphoma.
原发性干燥综合征（PSS）与淋巴增殖性恶性肿瘤的风险增加相关，B 细胞非霍奇金淋巴瘤（B-NHL）是最常见的类型。为了评估 PSS 患者中 CD4+T 淋巴细胞的分布以及 CD4+T 淋巴细胞减少与 B-NHL 发生的相关性，本研究纳入了 8 例 PSS 合并 B-NHL 的患者（I 组）、50 例无 B-NHL 的 PSS 患者（II 组）和 30 名健康志愿者作为对照组。使用流式细胞仪 Coulter EPICS-XL 评估循环 CD4+和 CD8+T 淋巴细胞分布以及 CD4+/CD8+T 细胞比值，并比较各组患者与对照组之间的差异。I 组 PSS 合并 B-NHL 患者的 CD4+T 淋巴细胞减少具有统计学意义，与无淋巴瘤患者和对照组相比（P=0.001）。此外，I 组的 CD4+/CD8+T 细胞比值显著低于 II 组和对照组（P=0.001）。还发现 CD4+T 淋巴细胞减少与其他危险因素（腮腺肿胀、血管炎、类风湿因子、低补体、冷球蛋白血症）呈显著正相关。CD4+T 淋巴细胞减少与 PSS 患者的 B-NHL 发生相关，可作为淋巴瘤的一个重要预测因子。
