University of Lyon, F-69622, Lyon, France.
J Biomed Nanotechnol. 2012 Oct;8(5):709-19. doi: 10.1166/jbn.2012.1439.
Temperature and glucose sensitive microgels were prepared via radical precipitation polymerization, using N-vinylcaprolactam (NVCL) as the main monomer, N,N-methylenebisacrylamide (MBA) as a crosslinker, V50 as initiator and 4-vinylphenylboronic acid (VPBA) as a functional monomer. The LCST of homopoly (NVCL) was first determined and found to be around 33 degrees C. The preparation of microgels was examined as a function of crosslinker (MBA) and VPBA amount in the polymerization recipe. Each polymerization was examined by considering the polymerization conversion and water-soluble polymer formation. Transmission electron microscopy, dynamic light scattering (DLS) and FT-IR characterizations show that the prepared microgels are monodisperse submicron particles, thermally sensitive and containing boronic acid functions. The effect of glucose on the swelling ability of boronic acid containing microgel was particularly examined and revealed the presence of specific interaction.
温度和葡萄糖敏感的微凝胶是通过自由基沉淀聚合制备的，使用 N-乙烯基己内酰胺（NVCL）作为主要单体，N，N-亚甲基双丙烯酰胺（MBA）作为交联剂，V50 作为引发剂，4-乙烯基苯硼酸（VPBA）作为功能单体。首先确定了均聚物（NVCL）的 LCST，发现其约为 33°C。通过考虑聚合转化率和水溶性聚合物形成，研究了微凝胶制备过程中交联剂（MBA）和 VPBA 用量的影响。通过透射电子显微镜、动态光散射（DLS）和傅里叶变换红外光谱（FT-IR）特性研究表明，所制备的微凝胶是单分散的亚微米颗粒，具有热敏感性和硼酸功能。特别研究了葡萄糖对含硼酸微凝胶溶胀能力的影响，揭示了存在特定相互作用。
