Biomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada.
Artif Cells Nanomed Biotechnol. 2013 Apr;41(2):116-24. doi: 10.3109/10731199.2012.712047. Epub 2012 Aug 14.
A new gut bacterial adhesion model has been developed. For this, a continuous-flow bioreactor packed with bacteria-coated beads was designed to simulate the gut lining and other features. In vitro model efficacy shows successful bacterial cell gut adhesions: bacterial adhesion was higher with mucin-alginate compared to controls. In feasibility study, adhesion of Lactobacillus fermentum NCIMB 5221 and Lactobacillus reuteri NCIMB 701359 was investigated for their metabolic activities for bile salt. Bile salt hydrolase (BSH)-active Lactobacillus reuteri exerted higher activity than non-BSH-active L. fermentum. This model has potential use in gut health, probiotic, bacterial cell gut adhesion and other delivery applications.
已经开发出一种新的肠道细菌黏附模型。为此，设计了一个装有细菌包被珠的连续流生物反应器来模拟肠道衬里和其他特征。体外模型功效显示出成功的细菌细胞肠道黏附：与对照相比，黏蛋白-海藻酸盐的细菌黏附更高。在可行性研究中，研究了发酵乳杆菌 NCIMB 5221 和罗伊氏乳杆菌 NCIMB 701359 的黏附及其对胆盐的代谢活性。具有胆盐水解酶 (BSH)活性的罗伊氏乳杆菌的活性高于无 BSH 活性的发酵乳杆菌。该模型在肠道健康、益生菌、细菌细胞肠道黏附和其他输送应用中具有潜在用途。
