School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, No.1 Wenyuan Road, Nanjing 210046, China.
National Center of Supervision Inspection on Processed Food &Food Additives Quality, Nanjing Institute of Product Quality Inspection, No.3 Jialingjiang East Street, Nanjing 210019, China.
FEMS Microbiol Lett. 2019 Apr 1;366(8). doi: 10.1093/femsle/fnz100.
Lactic acid bacteria (LAB) are major probiotics in food supplements. Survival in gastrointestinal (GI) tract is important for the effective use of LAB as probiotics. Bile salt hydrolase (BSH), which catalyzes the conversion of conjugated bile salts into free bile salts, can significantly modulate the gut microbiome. Here, we hypothesize that BSH is important for LAB survival and adhesion in the gut. The aim of this study is to investigate the effect of BSH on the survival of LAB in the GI tract. A panel of bsh genes from murine gut microbiota were amplified, cloned and expressed into Lactobacillus plantarum, which were then administered to mice by gavage. Our data indicated that the survival of BSH-positive L. plantarum was significantly prolonged in the GI tract compared with wild type L. plantarum. Furthermore, BSH-positive strains exhibited increased adhesion to Caco-2 intestinal cells than BSH-deleted L. plantarum. Enhanced adhesion to intestinal cells of BSH positive LAB can therefore be an important criterion for selecting effective probiotic strains in food industry.
乳酸菌（LAB）是食品补充剂中的主要益生菌。在胃肠道（GI）中生存对于 LAB 作为益生菌的有效利用非常重要。胆汁盐水解酶（BSH）可以催化结合胆汁盐转化为游离胆汁盐，从而显著调节肠道微生物组。在这里，我们假设 BSH 对 LAB 在肠道中的生存和黏附很重要。本研究旨在研究 BSH 对 LAB 在胃肠道中生存的影响。从鼠肠道微生物组中扩增、克隆和表达了一组 bsh 基因，并将其导入到植物乳杆菌中，然后通过灌胃将其施用于小鼠。我们的数据表明，与野生型植物乳杆菌相比，BSH 阳性植物乳杆菌在胃肠道中的存活时间明显延长。此外，BSH 阳性菌株对 Caco-2 肠细胞的黏附能力比 BSH 缺失的植物乳杆菌增强。因此，BSH 阳性 LAB 对肠道细胞的黏附增强可以成为食品工业中选择有效益生菌菌株的重要标准。
