Faculty of Science, Department of Chemistry, Ankara University, Tandoğan, Ankara, Turkey.
Artif Cells Nanomed Biotechnol. 2013 Jun;41(3):165-71. doi: 10.3109/10731199.2012.712045. Epub 2012 Aug 14.
Two new amperometric carbon paste enzyme electrodes including Fe(3)O(4) nanoparticles with and without 1,4-benzoquinone were developed for glucose determination. Electron transfer properties of unmodified and Fe(3)O(4) nanoparticles and/or 1,4-benzoquinone modified carbon paste electrodes were investigated in 0.1 M KCl support electrolyte containing Fe(CN)6(3-/4-) as redox probe by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. Fe(3)O(4) nanoparticles increased electron transfer at solution/electrode interface. The parameters affecting the analytical performance of the enzyme electrode have been investigated in detail and optimized for Fe(3)O(4) nanoparticle modified enzyme electrode (Fe(3)O(4)-CPEE). Fe(3)O(4) nanoparticles and 1,4-benzoquinone modified enzyme electrode (BQ-Fe(3)O(4)-CPEE) exhibited linear response from 1.9 × 10(-7) M to 3.7 × 10(-6) M, from 7.2 × 10(-6) M to 1.5 × 10(-4) M and from 1.3 × 10(-3) M to 1.2 × 10(-2) M with an excellent detection limit of 1.9 × 10(-8) M. BQ-Fe(3)O(4)-CPEE was used for determination of glucose in serum samples and results were in good agreement with those obtained by spectrophotometric method.
两种新的安培碳糊酶电极，包括含有和不含有 1,4-苯醌的 Fe(3)O(4)纳米粒子，被开发用于葡萄糖的测定。在含有 Fe(CN)6(3-/4-)作为氧化还原探针的 0.1 M KCl 支持电解质中，通过循环伏安法（CV）和电化学阻抗谱（EIS）方法研究了未修饰和 Fe(3)O(4)纳米粒子和/或 1,4-苯醌修饰的碳糊电极的电子转移性质。Fe(3)O(4)纳米粒子增加了溶液/电极界面的电子转移。详细研究了影响酶电极分析性能的参数，并对 Fe(3)O(4)纳米粒子修饰酶电极（Fe(3)O(4)-CPEE）进行了优化。Fe(3)O(4)纳米粒子和 1,4-苯醌修饰的酶电极（BQ-Fe(3)O(4)-CPEE）在 1.9 × 10(-7) M 至 3.7 × 10(-6) M、7.2 × 10(-6) M 至 1.5 × 10(-4) M 和 1.3 × 10(-3) M 至 1.2 × 10(-2) M 范围内呈现线性响应，检测限为 1.9 × 10(-8) M。BQ-Fe(3)O(4)-CPEE 用于血清样品中葡萄糖的测定，结果与分光光度法测定的结果吻合良好。
