Institute of Materials Science and Engineering, Henan Polytechnic University, Jiaozuo 454000, China; Cultivating Base for Key Laboratory of Environment-friendly Inorganic Materials in University of Henan Province, Henan Polytechnic University, Jiaozuo 454000, China.
Biosens Bioelectron. 2014 Jan 15;51:268-73. doi: 10.1016/j.bios.2013.07.054. Epub 2013 Aug 6.
Core-shell Fe3O4-enzyme-polypyrrole (Ppy) nanoparticles with excellent magnetism and conductivity were successfully prepared via the surface modification and enzyme self-encapsulation within Ppy. A novel potentiometric glucose biosensor has been constructed by effectively attaching the proposed Fe3O4-enzyme-Ppy nanoparticles to the surface of the magnetic glassy carbon electrode (MGCE). The optimum biosensing conditions could be provided with polymerization time of pyrrole for 6h and 0.42 mg immobilization amount of Fe3O4-enzyme-Ppy nanoparticles on MGCE. The performance of the developed glucose biosensor was evaluated and the results indicated that a sensitive glucose biosensor could be fabricated. The obtained glucose biosensor presents shorter response time (6 s), wider linear range (0.5 μM to 34 mM), lower limit of detection (LOD, 0.3 μM), high-selectivity monitoring of glucose and good stability (with about 98.1% of the initial response signal retained after 20 days). The analytical application of the glucose biosensor confirms the feasibility of glucose detection in serum sample.
核壳型 Fe3O4-酶-聚吡咯（Ppy）纳米粒子具有优异的磁性和导电性，通过 Ppy 内的表面修饰和酶自包封成功制备。通过有效将所提出的 Fe3O4-酶-Ppy 纳米粒子附着到磁性玻璃碳电极（MGCE）的表面，构建了一种新型的电位型葡萄糖生物传感器。最适的生物传感条件为吡咯聚合时间为 6h 和 MGCE 上固定化量为 0.42mg Fe3O4-酶-Ppy 纳米粒子。对开发的葡萄糖生物传感器的性能进行了评估，结果表明可以制备出灵敏的葡萄糖生物传感器。所得葡萄糖生物传感器具有较短的响应时间（6s）、较宽的线性范围（0.5μM 至 34mM）、较低的检测限（LOD，0.3μM）、对葡萄糖的高选择性监测和良好的稳定性（20 天后初始响应信号保留约 98.1%）。葡萄糖生物传感器的分析应用证实了血清样品中葡萄糖检测的可行性。
