使用具有质谱的差分离子淌度微芯片进行蛋白质分析。
Protein analyses using differential ion mobility microchips with mass spectrometry.
出版信息
Anal Chem. 2012 Sep 4;84(17):7297-300. doi: 10.1021/ac3018636. Epub 2012 Aug 13.
Abstract
Differential ion mobility spectrometry (FAIMS) integrated with mass spectrometry (MS) is a powerful new tool for biological and environmental analyses. Large proteins occupy regions of FAIMS spectra distinct from peptides, lipids, or other medium-size biomolecules, likely because strong electric fields align huge dipoles common to macroions. Here we confirm this phenomenon in separations of proteins at extreme fields using FAIMS chips coupled to MS and demonstrate their use to detect even minor amounts of large proteins in complex matrixes of smaller proteins and peptides.
摘要
差分离子迁移谱（FAIMS）与质谱（MS）联用是生物和环境分析的强大新工具。大蛋白质占据 FAIMS 光谱中与肽、脂质或其他中等大小生物分子不同的区域，这可能是因为强电场使大离子常见的巨大偶极子排列整齐。在这里，我们使用与 MS 耦合的 FAIMS 芯片在极端场中分离蛋白质时证实了这一现象，并证明它们可用于检测复杂的小蛋白质和肽混合物中即使是少量的大蛋白质。
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