United States Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Rd., Laurel, MD 20708, USA.
Food Chem Toxicol. 2012 Oct;50(10):3426-32. doi: 10.1016/j.fct.2012.07.055. Epub 2012 Aug 4.
Melamine and its triazine analogs, such as cyanuric acid, have been used to artificially inflate protein content both in animal feed ingredients, as well as in milk products produced for human consumption. We report here a LC-MS/MS method to quantify and confirm melamine and cyanuric acid in serum from channel catfish and rainbow trout with a limit of quantification of 0.8 μg/mL. The method was applied to serum samples from a residue depletion study in which fish were given a single oral dose of 20 mg/kg body weight melamine, cyanuric acid, or both compounds together. Samples were taken at 1, 3, 7, 14, and 28 days (an additional 42 day was added for trout). When given alone or in combination with cyanuric acid, melamine residues were highest on day 1 in both catfish and trout. Cyanuric acid was only quantifiable at day 1 in trout when given alone, and not at all in catfish. The serum half life of melamine in catfish was 1.50-1.62 days and 3.09-3.67 days in trout. This work highlights the differences of depletion kinetics in fish, which can be measured in days, as compared to the depletion in mammals, measured in hours.
三聚氰胺及其三聚氰胺类似物，如三聚氰酸，已被用于人为提高动物饲料成分和供人类食用的乳制品中的蛋白质含量。我们在此报告了一种 LC-MS/MS 方法，可定量和确证鲶鱼和虹鳟血清中的三聚氰胺和三聚氰酸，其定量限为 0.8 μg/mL。该方法应用于残留消除研究的血清样本中，其中鱼经口给予 20 mg/kg 体重的三聚氰胺、三聚氰酸或两者的混合物。在第 1、3、7、14 和 28 天（在虹鳟中增加了第 42 天）采集样本。当单独给予或与三聚氰酸一起给予时，在鲶鱼和虹鳟中，单独给予或与三聚氰酸一起给予时，三聚氰胺的残留量在第 1 天最高。当单独给予时，在虹鳟中仅在第 1 天可定量检测到三聚氰酸，而在鲶鱼中则根本无法检测到。鲶鱼中三聚氰胺的血清半衰期为 1.50-1.62 天，而在虹鳟中为 3.09-3.67 天。这项工作突出了鱼类消除动力学的差异，鱼类的消除动力学可以在几天内测量，而哺乳动物的消除动力学则可以在几小时内测量。
