Department of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan.
J Bioenerg Biomembr. 2012 Dec;44(6):629-34. doi: 10.1007/s10863-012-9466-8.
Mitochondria are essential organelles in eukaryotic cells and are responsible for regulating energy metabolism, ROS production, and cell survival. Recently, various cellular pathogeneses, including tumorigenesis and metastasis, have been reported to be associated with mitochondrial homeostasis. Consequently, exploiting the correlation between dysfunctional mitochondria and tumor progression has been implicated in the understanding of tumorigenesis, tumor metastasis, and chemoresistance, along with novel strategies to develop cancer therapeutics. To comprehensively understand the role of the mitochondria in cancer metastasis, it is necessary to resolve thousands of mitochondrial proteins and their post-translational modifications with high-throughput global assessments. We introduce mitochondrial proteomic strategies in this review and a discussion on their recent findings related to cancer metastasis. Additionally, the mitochondrial respiratory chain is believed to be a major site for ROS production, and elevated ROS is likely a key source to trigger dysfunctional mitochondria and impaired mitochondrial metabolism that subsequently contribute to the development of cancer progression. Equipment-based metabolomic analysis now allows the monitoring of disease progression and diagnosis. These newly emerging techniques, including proteomics, redox-proteomics, and metabolomics, are described in this review.
线粒体是真核细胞中的重要细胞器，负责调节能量代谢、ROS 生成和细胞存活。最近，已有多种细胞发病机制，包括肿瘤发生和转移，被报道与线粒体动态平衡有关。因此，利用功能失调的线粒体与肿瘤进展之间的相关性，有助于理解肿瘤发生、肿瘤转移和化疗耐药性，并为开发癌症治疗提供新策略。为了全面了解线粒体在癌症转移中的作用，有必要对数千种线粒体蛋白及其翻译后修饰进行高通量的全面评估。我们在这篇综述中介绍了线粒体蛋白质组学策略，并讨论了它们在癌症转移方面的最新发现。此外，线粒体呼吸链被认为是 ROS 生成的主要部位，而升高的 ROS 很可能是触发功能失调的线粒体和受损的线粒体代谢的关键来源，进而促进癌症进展的发展。基于设备的代谢组学分析现在可以监测疾病进展和诊断。本文综述了这些新兴技术，包括蛋白质组学、氧化还原蛋白质组学和代谢组学。
