a Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology , Jinan University , Guangzhou , China.
Expert Rev Proteomics. 2017 Oct;14(10):891-903. doi: 10.1080/14789450.2017.1374180. Epub 2017 Sep 7.
Mitochondria play important roles in regulating multiple biological processes and signalling pathways in eukaryotic cells, and mitochondrial dysfunction may result in a wide range of serious diseases, including cancer. With improvements in the identification of mitochondrial proteins, mitochondrial proteomics has made great achievements. In particular, this approach has been widely used to compare tumour cells at different stages of malignancy. Therefore, there is an urgent need to identify and characterize the function of mitochondrial proteins in cancer progression and to determine the involved mechanisms. Areas covered: We provide an overview of recent progress related to mitochondrial proteomics in cancer and the application of comparative mitochondrial proteomics in various biological processes, including apoptosis, necroptosis, autophagy and metastasis, as well as clinical progress in cancer. Proteomics-related reports were found using PubMed and Google Scholar databases. Expert commentary: Understanding both post-translational modification and post-translational processing is important in the comprehensive characterization of protein function. The application of comparative mitochondrial proteomics to investigate clinical samples and cancer cells will contribute to our understanding of the molecular interplay of mitochondrial proteins in the development of cancer. This approach will mine more biomarkers for diagnosis and prognosis and improve therapeutic outcomes among cancer patients.
线粒体在真核细胞中调节多种生物过程和信号通路方面发挥着重要作用，线粒体功能障碍可能导致广泛的严重疾病，包括癌症。随着对线粒体蛋白鉴定的改进，线粒体蛋白质组学取得了巨大的成就。特别是，这种方法已被广泛用于比较不同恶性程度阶段的肿瘤细胞。因此，迫切需要识别和表征线粒体蛋白在癌症进展中的功能，并确定相关机制。涵盖领域：我们提供了与癌症中线粒体蛋白质组学相关的最新进展概述，以及比较线粒体蛋白质组学在包括细胞凋亡、坏死性凋亡、自噬和转移在内的各种生物学过程中的应用，以及癌症的临床进展。使用 PubMed 和 Google Scholar 数据库查找蛋白质组学相关报告。专家评论：在全面描述蛋白质功能时，了解翻译后修饰和翻译后加工非常重要。将比较线粒体蛋白质组学应用于临床样本和癌细胞的研究将有助于我们理解线粒体蛋白在癌症发展过程中的分子相互作用。这种方法将挖掘更多用于诊断和预后的生物标志物，并改善癌症患者的治疗效果。
