Department of Oral Pathology, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea.
Photochem Photobiol. 2013 Jan-Feb;89(1):199-207. doi: 10.1111/j.1751-1097.2012.01225.x. Epub 2012 Sep 18.
Heat shock protein-27 (HSP27) is a member of the small HSP family which has been linked to the nuclear factor-kappa B (NF-κB) signaling pathway regulating inflammatory responses. Clinical reports have suggested that low-level light therapy/laser irradiation (LLLT) could be an effective alternative treatment to relieve inflammation during bacterial infection associated with periodontal disease. However, it remains unclear how light irradiation can modulate the NF-κB signaling pathway. We examined whether or not 635 nm irradiation could lead to a modulation of the NF-kB signaling pathway in HSP27-silenced cells and analyzed the functional cross-talk between these factors in NF-κB activation. The results showed that 635 nm irradiation led to a decrease in the HSP27 phosphorylation, reactive oxygen species (ROS) generation, I-κB kinase (IKK)/inhibitor of κB (IκB)/NF-κB phosphorylation, NF-κB p65 translocation and a subsequent decrease in the COX-1/2 expression and prostaglandin (PGE(2) ) release in lipopolysaccharide(LPS)-induced human gingival fibroblast cells (hGFs). However, in HSP27-silenced hGFs, no obvious changes were observed in ROS generation, IKK/IκB/NF-κB phosphorylation, NF-κB p65 translocation, nor in COX-1/2 expression, or PGE(2) release. This could be a mechanism by which 635 nm irradiation modulates LPS-induced NF-κB signaling pathway via HSP27 in inflammation. Thus, HSP27 may play a role in regulating the anti-inflammatory response of LLLT.
热休克蛋白 27（HSP27）是小分子热休克蛋白家族的一员，与核因子-κB（NF-κB）信号通路有关，该通路调节炎症反应。临床报告表明，低强度光疗法/激光照射（LLLT）可能是一种有效的替代治疗方法，可缓解与牙周病相关的细菌感染引起的炎症。然而，光照如何调节 NF-κB 信号通路仍不清楚。我们研究了 635nm 光照是否会导致 HSP27 沉默细胞中 NF-κB 信号通路的调节，并分析了 NF-κB 激活过程中这些因素之间的功能交叉对话。结果表明，635nm 光照导致 HSP27 磷酸化、活性氧（ROS）生成、I-κB 激酶（IKK）/κB 抑制剂（IκB）/NF-κB 磷酸化、NF-κB p65易位以及随后 COX-1/2 表达和前列腺素（PGE2）释放减少，在脂多糖（LPS）诱导的人牙龈成纤维细胞（hGFs）中。然而，在 HSP27 沉默的 hGFs 中，ROS 生成、IKK/IκB/NF-κB 磷酸化、NF-κB p65 易位以及 COX-1/2 表达或 PGE2 释放均无明显变化。这可能是 635nm 光照通过 HSP27 调节 LPS 诱导的 NF-κB 信号通路的一种机制，在炎症中。因此，HSP27 可能在调节 LLLT 的抗炎反应中起作用。
