Fagundes Mírian Ívens, Galvani Nathalia Coral, Generoso Camille M, De-Pieri Ellen, Scussel Rahisa, Fernandes Bruna B, Lima Igor Ramos, Abel Jéssica S, Corrêa Maria Eduarda A B, Possato Jonathann C, Feuser Paulo E, de Andrade Thiago A M, Chávez-Olortegui Carlos, Silveira Paulo Cesar Lock, Machado-de-Ávila Ricardo Andrez
Laboratory of Pathophysiology Experimental, Postgraduate Program in Health Sciences, Universidade do Extremo Sul Catarinense (UNESC), Criciúma, SC, Brazil.
Graduate Program of Biomedical Science, Herminio Ometto Foundation, Araras, 13607-339, Brazil.
Lasers Med Sci. 2025 Jan 29;40(1):53. doi: 10.1007/s10103-025-04300-6.
This study aimed to evaluate gold nanoparticles (GNPs) and photobiomodulation (PBM), associated with antibothropic serum (AS), to treat a muscle lesion induced by Bothrops jararaca venom.
108 Swiss male mice were used, divided into nine groups (n = 12) with different combinations of treatments. Animals were inoculated with 250 µg of B. jararaca venom at the right gastrocnemius muscle. The treatment started 12 h after the venom injection and occurred daily for 7 days. AS was administered as recommended by the Ministry of Health. Low-power aluminum gallium indium phosphide (AlGaInP) with continuous emission (wavelength 660 nm; average power 30 mW ± 20%; beam size of 0.06310 cm; and dose of 2 J for ± 1 min per point). Irradiation was applied to five distinct areas surrounding the wound, at a perpendicular angle to the skin. The groups treated with GNPs received treatment through the application of 1 mL of 20 nm GNPs (30 mg/L). Muscle, heart, and kidneys were removed for histological and biochemical analysis.
GNPs can improve the results achieved by PBM and the standard treatment, presenting significant benefits in histological parameters, such as the increase of new blood vessels, fibroblasts, collagen, and a reduction of inflammatory infiltrate.
GNPs can be considered an option to accelerate the recovery of Bothrops lesions.
本研究旨在评估与抗蛇血清（AS）联合使用的金纳米颗粒（GNP）和光生物调节（PBM）对由巴西矛头蝮蛇毒引起的肌肉损伤的治疗效果。
使用108只瑞士雄性小鼠，分为九组（n = 12），采用不同的治疗组合。在小鼠右腓肠肌接种250μg巴西矛头蝮蛇毒。在毒液注射后12小时开始治疗，每天进行，持续7天。按照卫生部的建议给予抗蛇血清。使用连续发射的低功率磷化铝镓铟（AlGaInP）（波长660nm；平均功率30mW±20%；光束尺寸0.063×1.0cm；每点剂量2J，照射±1分钟）。以与皮肤垂直的角度对伤口周围五个不同区域进行照射。接受金纳米颗粒治疗的组通过应用1mL 20nm金纳米颗粒（30mg/L）进行治疗。取出肌肉、心脏和肾脏进行组织学和生化分析。
金纳米颗粒可以改善光生物调节和标准治疗所取得的效果，在组织学参数方面呈现出显著益处，如新血管、成纤维细胞、胶原蛋白增加以及炎症浸润减少。
金纳米颗粒可被视为加速巴西矛头蝮蛇咬伤损伤恢复的一种选择。
