Wessels Quenton, Pretorius Etheresia, Smith Celeste M, Nel Hugo
Department of Anatomy, School of Medicine, University of Namibia, Windhoek, NamibiaDepartment of Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, South AfricaSouthern group of Companies, Southern Implants Office Park, Irene, South Africa.
Int Wound J. 2014 Apr;11(2):152-8. doi: 10.1111/j.1742-481X.2012.01052.x. Epub 2012 Aug 14.
Knowledge on the intrinsic mechanisms involved in wound healing provides opportunity for various therapeutic strategies. The manipulation of dermal fibroblast proliferation and differentiation might prove to beneficially augment wound healing. This study evaluated the combined effects of niacinamide, L-carnosine, hesperidin and Biofactor HSP(®) on fibroblast activity. The effects on fibroblast collagen production, cellular proliferation, migration and terminal differentiation were assessed. In addition, the authors determined the effects on in vitro wound healing. The optimal concentrations of actives were determined in vitro. Testing parameters included microscopic morphological cell analysis, cell viability and proliferation determination, calorimetric collagen detection and in vitro wound healing dynamics. Results show that 0·31 mg/ml niacinamide, 0·10 mg/ml L-carnosine, 0·05 mg/ml hesperidin and 5·18 µg/ml Biofactor HSP® proved optimal in vitro. The results show that fibroblast collagen synthesis was increased alongside with cellular migration and proliferation.
对伤口愈合所涉及的内在机制的了解为各种治疗策略提供了机会。对真皮成纤维细胞增殖和分化的调控可能会有益地促进伤口愈合。本研究评估了烟酰胺、L-肌肽、橙皮苷和生物因子热休克蛋白（Biofactor HSP®）对成纤维细胞活性的联合作用。评估了对成纤维细胞胶原蛋白产生、细胞增殖、迁移和终末分化的影响。此外，作者还确定了对体外伤口愈合的影响。在体外确定了活性成分的最佳浓度。测试参数包括显微镜下细胞形态分析、细胞活力和增殖测定、比色法胶原蛋白检测以及体外伤口愈合动力学。结果表明，0·31 mg/ml烟酰胺、0·10 mg/ml L-肌肽、0·05 mg/ml橙皮苷和5·18 µg/ml生物因子热休克蛋白（Biofactor HSP®）在体外被证明是最佳的。结果表明，成纤维细胞胶原蛋白合成随着细胞迁移和增殖而增加。
