Laboratory of Genetic Toxicity-TOXIGEN, Postgraduate Program in Genetics and Applied Toxicology-PPGGTA, Universidade Luterana do Brasil-ULBRA, Canoas, Brazil.
J Med Food. 2012 Oct;15(10):873-8. doi: 10.1089/jmf.2012.0032. Epub 2012 Aug 14.
Cynara scolymus L. (Asteraceae), popularly known as artichoke, has been widely used in herbal medicine for the treatment of hepatic diseases. The genotoxicity of C. scolymus L. leaf extract (LE) and the ability to modulate the genetic toxicity of the alkylating agent ethyl methanesulfonate (EMS) were assessed using the comet assay on Chinese hamster ovary cells. Genotoxicity was evaluated after 1- and 24-h treatments using four different LE concentrations: 0.62, 1.25, 2.5, and 5.0 mg/mL. Antigenotoxicity was assessed for pretreatment, simultaneous treatment, and post-treatment. All doses used led to a significant increase in the frequency of DNA damage, after exposure for 1 and 24 h. In the antigenotoxicity experiments, LE reduced the frequency of DNA damage induced by EMS in the simultaneous treatment only. However, the lowest dose was more protective than higher concentrations. Flavonoids and phenolic compounds are, probably, the C. scolymus constituents responsible for its genotoxic and antigenotoxic effects.
朝鲜蓟（Cynara scolymus L.）（菊科），俗称朝鲜蓟，在草药医学中广泛用于治疗肝脏疾病。使用彗星试验评估朝鲜蓟叶提取物（LE）的遗传毒性和调节烷化剂甲基甲烷磺酸盐（EMS）遗传毒性的能力，该试验在中华仓鼠卵巢细胞上进行。使用四种不同的 LE 浓度：0.62、1.25、2.5 和 5.0mg/mL，分别在 1 小时和 24 小时处理后评估遗传毒性。对预处理、同时处理和后处理进行抗原毒性评估。在暴露 1 和 24 小时后，所有使用的剂量均导致 DNA 损伤频率显著增加。在抗原毒性实验中，LE 仅在同时处理时降低 EMS 诱导的 DNA 损伤频率。然而，最低剂量比高浓度更具保护作用。类黄酮和酚类化合物可能是朝鲜蓟具有遗传毒性和抗原毒性作用的成分。
