a Department of Medical Biochemistry, Faculty of Pharmacy , University of Belgrade , Belgrade , Serbia.
b Department of Clinical Chemistry, Faculty of Pharmacy , University of Ljubljana , Ljubljana , Slovenia.
Pharm Biol. 2018 Dec;56(1):138-144. doi: 10.1080/13880209.2018.1434549.
Polyphenols and flavonoids in artichoke leaf tincture (ALT) protect cells against oxidative damage.
We examined ALT effects on deoxyribonucleic acid (DNA) damage and lipid profiles in rat plasma and gene expression in rat aorta [haemeoxygenase-1 (HO1), haemeoxygenase-2 (HO2), NADPH oxidase 4 (NOX-4), monocyte chemoattractant protein-1 (MCP-1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2)].
Eighteen male Wistar albino rats were divided into three groups (n = 6/group): The control group (CG) was fed with standard pellet chow for 11 weeks; the AD group was fed for a similar period of time with pellet chow supplemented with 2% cholesterol, 3% sunflower oil and 1% sodium cholate. The ADA group was fed with pellet chow (for 1 week), the atherogenic diet (see above) for the following 4 weeks and then with ALT (0.1 mL/kg body weight) and atherogenic diet for 6 weeks. According to HPLC analysis, the isolated main compounds in ALT were chlorogenic acid, caffeic acid, isoquercitrin and rutin.
Normalized HO-1 [0.11 (0.04-0.24)] and MCP-1 [0.29 (0.21-0.47)] mRNA levels and DNA scores [12.50 (4.50-36.50)] were significantly lower in the ADA group than in the AD group [0.84 (0.35-2.51)], p = 0.021 for HO-1 [0.85 (0.61-3.45)], p = 0.047 for MCP-1 and [176.5 (66.50-221.25)], p = 0.020 for DNA scores. HO-1 mRNA was lower in the ADA group than in the CG group [0.30 (0.21-0.71), p = 0.049].
Supplementation with ALT limited the effects of the atherogenic diet through reduced MCP-1 expression, thereby preventing oxidative damage.
朝鲜蓟叶酊中的多酚和类黄酮可保护细胞免受氧化损伤。
我们研究了 ALT 对大鼠血浆中脱氧核糖核酸（DNA）损伤和脂质谱以及大鼠主动脉中基因表达的影响[血红素加氧酶-1（HO1）、血红素加氧酶-2（HO2）、NADPH 氧化酶 4（NOX-4）、单核细胞趋化蛋白-1（MCP-1）和核因子（红细胞衍生 2）样 2（Nrf2）]。
将 18 只雄性 Wistar 白化大鼠分为三组（每组 6 只）：对照组（CG）用标准颗粒饲料喂养 11 周；AD 组用颗粒饲料喂养相同时间，饲料中添加 2%胆固醇、3%葵花籽油和 1%胆酸钠。ADA 组先喂食颗粒饲料（1 周），再喂食致动脉粥样硬化饲料（见上文）4 周，然后喂食 ALT（0.1ml/kg 体重）和致动脉粥样硬化饲料 6 周。根据 HPLC 分析，ALT 中分离出的主要化合物为绿原酸、咖啡酸、异槲皮苷和芦丁。
与 AD 组相比，ADA 组的 HO-1[0.11（0.04-0.24）]和 MCP-1[0.29（0.21-0.47）]mRNA 水平和 DNA 评分[12.50（4.50-36.50）]显著降低，p=0.021 用于 HO-1[0.85（0.61-3.45）]，p=0.047 用于 MCP-1 和[176.5（66.50-221.25）]，p=0.020 用于 DNA 评分。ADA 组的 HO-1mRNA 水平低于 CG 组[0.30（0.21-0.71）]，p=0.049。
ALT 的补充通过降低 MCP-1 的表达来限制致动脉粥样硬化饮食的作用，从而防止氧化损伤。
