Department of Pathology and Infectious Diseases, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK.
Res Vet Sci. 2013 Feb;94(1):22-6. doi: 10.1016/j.rvsc.2012.07.024. Epub 2012 Aug 11.
Transmission of Actinobacillus pleuropneumoniae is primarily thought to be via direct transfer of mucus from pig to pig. For transfer between farms, the organism may need to persist in the wet or dried state to be carried on an inanimate surface. The survival of A. pleuropneumoniae was investigated under controlled laboratory conditions. In aqueous suspension, survival was improved by the presence of NaCl and mucin; it was prolonged at lower temperature. In dry state, it survived best on a hydrophobic surface either under desiccated conditions or saturated humidity. Detectable viability was maintained for 3-4 days. When frozen, A. pleuropneumoniae survived for more than 17 weeks at -20 °C, but the viability declined to 0.01% during that time. Survival at -70 °C was effective for long term storage. Results obtained from this investigation would be applicable for sampling method, transport techniques, epidemiological study, and biosecurity implementation.
胸膜肺炎放线杆菌的传播主要被认为是通过猪与猪之间直接转移粘液。对于农场之间的转移，该生物体可能需要在潮湿或干燥状态下存活，以便在无生命的表面上传播。在受控的实验室条件下研究了胸膜肺炎放线杆菌的存活情况。在水悬浮液中，存在 NaCl 和粘蛋白可提高存活率；在较低温度下，存活时间延长。在干燥状态下，它在疏水性表面上的存活最佳，无论是在干燥条件下还是在饱和湿度下。可检测的存活能力可维持 3-4 天。当冷冻时，胸膜肺炎放线杆菌在-20°C 下可存活超过 17 周，但在此期间其活力下降到 0.01%。-70°C 的存活对长期储存有效。该研究的结果可适用于采样方法、运输技术、流行病学研究和生物安全实施。
